Nataša Resnik (Avtor), Tim Prezelj (Avtor), Giulia M. R. de Luca (Avtor), Erik Manders (Avtor), Roman S. Polishchuk (Avtor), Peter Veranič (Avtor), Mateja Erdani-Kreft (Avtor)

Povzetek

Tunneling membrane nanotubes (TnTs) are membrane protrusions connecting nearby or distant cells in vitro and in vivo. Functions of TnTs in cellular processes are various and rely on TnT structure, which also depends on cytoskeletal composition. In the present study, we focused on the organization of microtubules (MTs) and intermediate filaments (IFs) in TnTs of urothelial cells. We analysed TnTs of normal porcine urothelial cells, which morphologically and physiologically closely resemble normal human urothelial cells, and of cancer cells derived from invasive human urothelial neoplasm. Wide-field fluorescence, confocal and super-resolution microscopy techniques, together with image analyses and 3D reconstructions enlightened specific MT-IF organization in TnTs, and for the first time revealed that MTs and IFs co-occur in the majority of normal and cancer urothelial cell TnTs. Our findings show that in the initiation segment of TnTs, MTs are cross-linked with each other into filamentous network, however in the middle and the attaching segment of TnT, MTs can helically enwrap IFs, the phenomenon that has not been shown before within the TnTs. In this study, we assess MT-IF co-occurrence in TnTs and present evidence that such helical organization of MTs enwrapping IFs is only occurring in a minority of the TnTs. We also discuss the possible cell-biological and physiological reasons for helical organization of MTs in TnTs.

Ključne besede

microtubules;cancer urothelial cells;tunneling membrane nanotubes;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL MF - Medicinska fakulteta
UDK: 616.6
COBISS: 34052057 Povezava se bo odprla v novem oknu
ISSN: 2045-2322
Št. ogledov: 309
Št. prenosov: 101
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: mikrotubulari;rakave urotelijske celice;tunelske membranske nanocevke;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-8
Zvezek: ǂVol. ǂ8
Čas izdaje: Nov. 2018
DOI: 10.1038/s41598-018-35370-y
ID: 13181168