Ramila Mammadova (Avtor), Immacolata Fiume (Avtor), Ramesh Bokka (Avtor), Veronika Kralj-Iglič (Avtor), Darja Božič (Avtor), Matic Kisovec (Avtor), Marjetka Podobnik (Avtor), Apolonija Bedina Zavec (Avtor), Matej Hočevar (Avtor), Gabriella Gellén (Avtor), Gitta Schlosser (Avtor), Gabriella Pocsfalvi (Avtor)

Povzetek

Plant-derived nanovesicles (NVs) have attracted interest due to their anti-inflammatory, anticancer and antioxidative properties and their efficient uptake by human intestinal epithelial cells. Previously we showed that tomato (Solanum lycopersicum L.) fruit is one of the interesting plant resources from which NVs can be obtained at a high yield. In the course of the isolation of NVs from different batches of tomatoes, using the established differential ultracentrifugation or size-exclusion chromatography methods, we occasionally observed the co-isolation of viral particles. Density gradient ultracentrifugation (gUC), using sucrose or iodixanol gradient materials, turned out to be efficient in the separation of NVs from the viral particles. We applied cryogenic transmission electron microscopy (cryo-TEM), scanning electron microscopy (SEM) for the morphological assessment and LC–MS/MS-based proteomics for the protein identification of the gradient fractions. Cryo-TEM showed that a low-density gUC fraction was enriched in membrane-enclosed NVs, while the high-density fractions were rich in rod-shaped objects. Mass spectrometry–based proteomic analysis identified capsid proteins of tomato brown rugose fruit virus, tomato mosaic virus and tomato mottle mosaic virus. In another batch of tomatoes, we isolated tomato spotted wilt virus, potato virus Y and southern tomato virus in the vesicle sample. Our results show the frequent co-isolation of plant viruses with NVs and the utility of the combination of cryo-TEM, SEM and proteomics in the detection of possible viral contamination.

Ključne besede

paradižnik;rastlinski virusi;virus paradižnikove rjave rugoze;virus paradižnikovega mozaika;paradižnikova pegavost;zunajcelični vezikli;kapsidna beljakovina;tomato;plant viruses;tomato brown rugose fruit virus;tomato mosaic virus;tomato spotted wilt virus;extracellular vesicles;capsid protein;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: IMT - Inštitut za kovinske materiale in tehnologije
UDK: 577
COBISS: 71549443 Povezava se bo odprla v novem oknu
ISSN: 2079-4991
Št. ogledov: 417
Št. prenosov: 79
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Vrsta dela (COBISS): Članek v reviji
Strani: 1-19
Letnik: ǂiss. ǂ8
Zvezek: ǂ[article] ǂ1922
Čas izdaje: Aug. 2021
DOI: 10.3390/nano11081922
ID: 13181177