Vsebnost flavonoidov v različnih delih tatarske ajde

magistrsko delo

Miha Medvešek (Avtor), Ivan Kreft (Recenzent), Mateja Germ (Mentor), Mateja Germ (Član komisije za zagovor), Ivan Kreft (Član komisije za zagovor), Matevž Likar (Član komisije za zagovor)

Povzetek

Navadno ajdo (Fagopyrum esculentum Moench) in tatarsko ajdo (Fagopyrum tataricum (L.) Gaertn.) smo gojili na petih lokacijah z nadmorskimi višinami med 300 in 1200 metrov. Tekom rastne sezone smo izvedli štiri vzorčenja. V vzorcih prvega, pred cvetenjem, in drugega vzorčenja, med cvetenjem, smo izmerili optične (presevnost in odbojnost) in biokemijske lastnosti listov (vsebnost UV absorbirajočih snovi, klorofila a, klorofila b, karotenoidov, antocianov, rutina in kvercetina). V vzorcih 3. in 4. vzorčenja, ki smo ju izvedli v zamiku približno desetih dni, ob koncu rastne sezone, pa smo izmerili višino rastlin, suho maso stebel, listov in semen ter vsebnost rutina in kvercetina v listih in semenih.Vsebnost rutina v listih obeh vrst je visoka in primerljiva. Najvišjo vsebnost rutina v listih smo pri obeh vrstah izmerili v času cvetenja. Semena (oreški) tatarske ajde vsebujejo približno 50-krat več rutina na g suhe snovi od semen navadne ajde. Semena obeh vrst vsebujejo več rutina v notranjosti semena, manj pa v luščini. Vsebnost rutina in kvercetina v delih navadne in tatarske ajde je vsaj deloma pogojena z nadmorsko višino uspevanja. Z nadmorsko višino se višata količina UV-B sevanja ter, predvsem v zgodnje poletnem in jesenskem času, pojavnost nizkih temperatur. Rast rastlin v nižini je omejevala predvsem suša na vrhuncu poletja. Rastline tatarske ajde izkazujejo višjo stopnjo tolerance na sušne razmere od rastlin navadne ajde. Vsebnost rutina ob koncu rastne sezone (ob spravilu pridelka) v listih tatarske ajde se z nadmorsko višino zvišuje. Enako velja za vsebnost rutina v listih navadne ajde iz poskusnih lokacij na 300, 600 in 850 metrih nadmorske višine. Na lokacijah z višjo nadmorsko višino je bila vsebnost rutina v listih nižja, kar nakazuje na to, da so okoljski dejavniki na visoko ležečih lokacijah (1085 in 1200 m n. m. v.) presegli zmožnost odziva (mejo tolerančnega območja) rastlin navadne ajde. Na vsebnost kvercetina v delih navadne in tatarske ajde pozitivno vplivajo nizke temperature. Na dvig vsebnosti kvercetina v listih tatarske ajde je vplivalo tudi objedanje srn.

Ključne besede

navadna ajda;tatarska ajda;rutin;kvercetin;flavonoidi;nadmorska višina;

Podatki

Jezik:	Slovenski jezik
Leto izida:	2021
Tipologija:	2.09 - Magistrsko delo
Organizacija:	UL BF - Biotehniška fakulteta
Založnik:	[M. Medvešek]
UDK:	581.5(043.2)
COBISS:	75104259
Št. ogledov:	273
Št. prenosov:	44
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Angleški jezik
Sekundarni naslov:	Flavonoid content in different parts of Tartary buckwheat
Sekundarni povzetek:	Common buckwheat (Fagopyrum esculentum Moench) and Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) were grown on five locations with different elevations ranging between 300 and 1200 m above sea level. Plants were sampled four times during the growing season. On the leaves from first, pre-flowering period, and second sampling, flowering period, pigments content (UV absorbing compounds, Chlorophyll a, Chlorophyll b, carothenoids, anthocyanins, rutin and quercetin) and optical properties were measured. 3rd and 4th sampling were conducted in time span of roughly ten days at the end of growing period during harvesting. Plants height, dry steam, leaves and seed (achenes) mass, rutin and quercetin concentration in leaves and seeds were measured. Rutin content in leaves of both Tartary and common buckwheat is high and does not differ much between the two species. The highest rutin content in leaves of both species was observed during the flowering period. Seeds of Tartary buckwheat contain roughly 50-times higher concentration of rutin than common buckwheat seeds. Rutin and quercetin content in different parts of common and Tartary buckwheat are, at least to certain extent, positively affected by altitude. Among all the environmental factors that vary with altitude, lower temperature, especially at the beginning and at the end of the season, and high radiation are the most important factors limiting the plants growth. In the low lands plant growth was decompromised by the lack of precipitation at the height of summer. Rutin concentrations also exhibit a role in plants reaction to dehydration stress. Tartary buckwheat plants seems to cope with drought conditions better that common buckwheat plants. At the end of the growing season, in the case of common buckwheat, sudden drop in rutin concentration was observed on locations with high altitude (1085 and 1200 m a. s. l.). This suggests that stress conditions on locations with high altitude exceeded plants ability to adapt to environmental stress.
Sekundarne ključne besede:	common buckwheat;Tartary buckwheat;rutin;quercetin;flavonoids;altitude;
Vrsta dela (COBISS):	Magistrsko delo/naloga
Študijski program:	0
Konec prepovedi (OpenAIRE):	1970-01-01
Komentar na gradivo:	Univ. Ljubljana, Biotehniška fak.
Strani:	XIII, 108, [1] str.
ID:	13307394