Katarina Miš (Avtor), Zoran Grubič (Avtor), Paola Lorenzon (Avtor), Marina Sciancalepore (Avtor), Tomaž Marš (Avtor), Sergej Pirkmajer (Avtor)

Povzetek

Acetylcholinesterase (AChE) and agrin, a heparan-sulfate proteoglycan, reside in the basal lamina of the neuromuscular junction (NMJ) and play key roles in cholinergic transmission and synaptogenesis. Unlike most NMJ components, AChE and agrin are expressed in skeletal muscle and [alpha]-motor neurons. AChE and agrin are also expressed in various other types of cells, where they have important alternative functions that are not related to their classical roles in NMJ. In this review, we first focus on co-cultures of embryonic rat spinal cord explants with human skeletal muscle cells as an experimental model to study functional innervation in vitro. We describe how this heterologous rat-human model, which enables experimentation on highly developed contracting human myotubes, offers unique opportunities for AChE and agrin research. We then highlight innovative approaches that were used to address salient questions regarding expression and alternative functions of AChE and agrin in developing human skeletal muscle. Results obtained in co-cultures are compared with those obtained in other models in the context of general advances in the field of AChE and agrin neurobiology.

Ključne besede

acetylcholinesterase;human muscle;apoptosis;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.02 - Pregledni znanstveni članek
Organizacija: UL MF - Medicinska fakulteta
UDK: 616-092
COBISS: 33379545 Povezava se bo odprla v novem oknu
ISSN: 1420-3049
Št. ogledov: 258
Št. prenosov: 59
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: acetilholinesteraza;človeška mišica;apoptoza;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-30
Letnik: ǂVol. ǂ22
Zvezek: ǂiss. ǂ9
Čas izdaje: 2017
DOI: 10.3390/molecules22091418
ID: 13505855