diplomsko delo
Lana Staver (Avtor), Peter Bohinc (Recenzent), Klemen Bohinc (Mentor), Anže Abram (Komentor), Franc Rojko (Komentor)

Povzetek

Uvod: V oralni mikroflori se ne moremo izogniti mikroorganizmom, ki se nabirajo v obliki biofilma. Bakterija, ki je najbolj povezana s tvorbo plaka, je Streptococcus mutans, ki je navadno prisotna v ustni votlini. Na bakterijsko adhezijo in tvorbo biofilma vplivajo različni okoljski dejavniki in fizikalno-kemične lastnosti materialov. V zobni protetiki se kot zobni nadomestek vedno bolj uporablja cirkon oksidna keramika, saj ima odlične mehanske in estetske lastnosti ter je biokompatibilna. Namen: Namen je dokazati, kako različna sestava in različna obdelava površine vpliva na adhezijo bakterije Streptococcus mutans in razvoj biofilma. Preverjali bomo, kako nekatere fizikalne lastnosti kot so pretočni potencial, hrapavost ter kontaktni kot površin vplivajo na adhezijo te bakterije. Metode dela: Pripravili smo vzorce štirih granul oz. sestav keramike, ki se uporabljajo za izdelavo zobnih kron, inlejev in onlejev. Primerjali smo cirkon oksidno keramiko z različno vsebnostjo dodatkov, in sicer 3 mol% Y2O3 (TZ- 3YB-E), 4 mol% Y2O3 (Zpex 4), 5,2 mol% Y2O3 (Zpex Smile) in 0,05 mol% Al2O3 (TZ-PX-242A). Pripravo ploščic smo nadaljevali s tem, da smo jih sintrali. Polovico sintranih ploščic smo tudi peskali. Sledila je polna karakterizacija površin za analizo fizikalnih lastnosti, kot so hrapavost površine, zeta potencial površine in kontaktni kot. V mikrobiološkem laboratoriju smo pripravili čisto kulturo Streptococcus mutans ter na vzorcih različnih sestav in obdelav raziskovali adhezijo te bakterije. V zobnem laboratoriju smo pripravili ZrO2 monolitne polnokeramične zobne prevleke na zobeh 11, 12, 21 in 22 s tehnologijo CAD-CAM. Rezultati: Največjo hrapavost smo izmerili pri peskanih površinah. Pri cirkonijevem oksidu stabiliziranim z itrijevim oksidom opazimo, da z večanjem deleža itrijevega oksida hrapavost površine pada. Najmanj negativni naboj smo izmerili na površini Zpex4 p (-22 mV ± 12 mV), najbolj negativen pa pri cirkonijevim oksidom stabiliziranim s 3 mol% Y2O3 (TZ- 3YB-E) as (-56 mV ± 3 mV). Peskane površine so manj negativno nabite kot samo sintrane, razen pri sestavi TZ-PX-242A (ZrO2 + 0,05 mol% Al2O3). Najnižji kontaktni kot (62,6°) je bil izmerjen pri peskani površini Zpex4, najvišji (87,8°) pa pri sintranem Zpex-Smile. Površine, ki so bile samo sintrane, so imele višji kontaktni kot tiste, ki so bile peskane, razen pri cirkonijevim oksidom stabiliziranim s 3 mol% Y2O3 (TZ- 3YB-E). Najvišjo absorbanco smo izmerili pri površini Zpex-Smile p (0,36 ± 0,04), najnižjo pa pri Zpex-Smile as (0,09 ± 0,00). Za tri od štirih sestav velja, da smo izmerili višjo absorbanco pri peskanih površinah kot pri tistih, ki so samo sintrane. Razprava in zaključek: Dejavnik, ki je najbolj vplival na adhezijo bakterij, je hrapavost površine. Potrdili smo tudi, da bolj negativen pretočni potencial površine pomeni manjšo stopnjo adhezije bakterije S. mutans. Zaradi nejasnih rezultatov ne moremo točno določiti, v kolikšni meri hidrofobnost vpliva na adhezijo bakterij.

Ključne besede

diplomska dela;laboratorijska zobna protetika;Y-TZP;Streptococcus mutans;adhezija bakterij;fizikalne lastnosti;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.11 - Diplomsko delo
Organizacija: UL ZF - Zdravstvena fakulteta
Založnik: [L. Staver]
UDK: 617.3
COBISS: 78949891 Povezava se bo odprla v novem oknu
Št. ogledov: 319
Št. prenosov: 133
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Adhesion of the bacteria Streptococcus mutans on zirconium oxide with additives
Sekundarni povzetek: Introduction: In oral microflora, microorganisms that pile in the form of biofilm cannot be avoided. The bacterium most associated with plaque formation is Streptococcus mutans, which is commonly present in the oral cavity. Bacterial adhesion and biofilm formation are affected by various environmental factors and physico-chemical properties of bacterial and material surfaces. In dental prosthetics, zirconium oxide ceramics is increasingly used as a dental substitute, as it has excellent mechanical and aesthetic properties and is biocompatible. Purpose: The aim of this study is to evaluate how different composition and different surface treatment affect Streptococcus mutans adhesion and biofilm formation. We will examine how certain physical properties such as streaming potential, surface roughness and hydrophobicity of the material surface influence the degree of adhesion of Streptococcus mutans. Methods: We prepared samples of four granules used for production of dental crowns, inlays and onlays. Zirconium oxide ceramics with different content of additives were compared, namely 3 mol% Y2O3 (TZ- 3YB-E), 4 mol% Y2O3 (Zpex 4), 5,2 mol% Y2O3 (Zpex Smile) and 0,05 mol% Al2O3 (TZ-PX-242A). We continued the preparation of the discs by sintering them. Half of the sintered discs were also sanded. This was followed by complete characterization for the analysis of physical properties such as surface roughness, zeta surface potential and hydrophobicity/contact angle. We prepared a pure culture of Streptococcus mutans in the microbiological laboratory and investigated the adhesion of this bacterium on discs of various compositions and treatments. In the dental laboratory we made monolithic ZrO2 ceramic dental veneers on teeth 11, 12, 21 and 22 with CAD-CAM technology. Results: The highest roughness was measured on sanded surfaces. In the case of zirconium oxide stabilized with yttrium oxide, it is observed that the surface roughness decreases with increasing proportion of yttrium oxide. The least negative charge was measured on the surface of Zpex4 p (-22 mV ± 12 mV) and the most negative in zirconium oxide stabilized with 3mol % Y2O3 (TZ- 3YB-E) as (-56 mV ± 3 mV). Sanded surfaces are less negatively charged than only sintered, except in the composition TZ-PX-242A (ZrO2 + 0,05 mol% Al2O3). The lowest contact angle (62,6°) was measured on sanded Zpex4 surface and the highest (87,8°) on as sintered Zpex-smile. Surfaces that were only sintered had higher contact angle than those that were sanded, except for zirconium oxide stabilized with 3 mol% Y2O3 (TZ- 3YB-E). The highest absorbance was measured at the Zpex-Smile p surface (0,36 ± 0,04) and the lowest on the Zpex-Smile as (0,09 ± 0,00). Three of the four materials we measured there was higher absorbance in sanded surfaces than in those that are only sintered. Discussion and conclusion: The factor, that most affected the bacterial adhesion is surface roughness. We also confirmed that a more negative streaming potential means lower degree of adhesion of Streptococcus mutans. Due to unclear results, it is not possible to determine exactly to what extent hydrophobicity affects bacterial adhesion.
Sekundarne ključne besede: diploma theses;laboratory dental prosthetics;Y-TZP;Streptococcus mutans;bacterial adhesion;physical properties;
Vrsta dela (COBISS): Diplomsko delo/naloga
Študijski program: 0
Komentar na gradivo: Univ. v Ljubljani, Zdravstvena fak., Oddelek za laboratorijsko zobno protetiko
Strani: 33 str., [4] str. pril.
ID: 13634762