Povzetek
The aim of this study was to characterize the properties of lactoferrin (LF) obtained in a process developed for its isolation from acid whey derived from the production of fresh curd cheese, using a unique technology of ion-exchange chromatography on CIM$^®$ monolithic columns. The freeze-dried lactoferrin samples produced on the pilot plant (capacity 1 m$^3$) were examined for the purity, iron-binding capacity, antibacterial activity, and pH- and temperature-stability. Apo-LF inhibited several tested strains (enterobacteria, Staphylococcus, Streptococcus salivarius) except clostridia, lactic acid bacteria, and bifidobacteria. Sample of LF intentionally saturated with Fe$^{3+}$ lost its antibacterial activity, indicating the involvement of mechanisms based on depriving bacteria of an iron source. All samples, regardless of the iron-saturation level, exhibited stability in pH range 4.0 to 11.0. LF with higher iron content (A-value = 41.9%) showed better thermal stability. Heat treatment up to 72 °C/3 s did not reduce antimicrobial activity against E. coli O157: H7 tox-. Higher purity (above 91%), higher iron-binding capacity and higher inhibitory activity against E. coli O157: H7 tox- compared to some similar products from the market was observed. These results demonstrate a high potential of monolithic ion-exchange chromatography for industrial processing of acid whey as a source of LF that can be used in new products with high-added value. The upscaling of the process is ongoing on a demonstration plant (10–30 m$^3$/day capacity).
Ključne besede
mikrobiologija;mlekarstvo;kisla sirotka;laktoferin;protimikrobno delovanje;ionska izmenjevalna kromatografija;
Podatki
Jezik: |
Angleški jezik |
Leto izida: |
2020 |
Tipologija: |
1.01 - Izvirni znanstveni članek |
Organizacija: |
UL BF - Biotehniška fakulteta |
UDK: |
579.67:637.1:543.544.14 |
COBISS: |
23698947
|
ISSN: |
2227-9717 |
Št. ogledov: |
245 |
Št. prenosov: |
91 |
Ocena: |
0 (0 glasov) |
Metapodatki: |
|
Ostali podatki
Sekundarni jezik: |
Slovenski jezik |
Sekundarne ključne besede: |
mikrobiologija;mlekarstvo;kisla sirotka;laktoferin;protimikrobno delovanje;ionska izmenjevalna kromatografija; |
Vrsta dela (COBISS): |
Članek v reviji |
Strani: |
str. 1-19 |
Letnik: |
ǂVol. ǂ8 |
Zvezek: |
ǂart. no. ǂ804 |
Čas izdaje: |
2020 |
DOI: |
10.3390/pr8070804 |
ID: |
13805840 |