Nada Kraševec (Avtor), Maruša Novak (Avtor), Simona Barat (Avtor), Matej Skočaj (Avtor), Kristina Sepčić (Avtor), Gregor Anderluh (Avtor)

Povzetek

Aegerolysins are small lipid-binding proteins particularly abundant in fungi. Aegerolysins from oyster mushrooms interact with an insect-specific membrane lipid and, together with MACPF proteins produced by the same organism, form pesticidal pore-forming complexes. The specific interaction with the same membrane lipid was recently demonstrated for nigerolysin A2 (NigA2), an aegerolysin from Aspergillus niger. In Aspergillus species, the aegerolysins were frequently found as secreted proteins, indicating their function in fungal defense. Using immunocytochemistry and live-cell imaging we investigated the subcellular localization of the nigerolysins A in A. niger, while their secretion was addressed by secretion prediction and Western blotting. We show that both nigerolysins A are leaderless proteins that reach the cell exterior by an unconventional protein secretion. NigA proteins are evenly distributed in the cytoplasm of fungal hyphae. A detailed bioinformatics analysis of Aspergillus aegerolysins suggests that the same function occurs only in a limited number of aegerolysins. From alignment, analysis of chromosomal loci, orthology, synteny, and phylogeny it follows that the same or a similar function described for pairs of pesticidal proteins of Pleurotus sp. can be expected in species of the subgenus Circumdati, section Nigri, series Nigri, and some other species with adjacent pairs of putative pesticidal proteins.

Ključne besede

egerolizin;nigerolizin;MACPF-protein;Aspergillus;glive;bioinformatika;lokalizacija;nekonvencionalna sekrecija;ceramid fosfoetanolamin;vezava na nevretenčarske lipide;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 577
COBISS: 42464771 Povezava se bo odprla v novem oknu
ISSN: 2076-2607
Št. ogledov: 155
Št. prenosov: 53
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: egerolizin;nigerolizin;MACPF-protein;Aspergillus;glive;bioinformatika;lokalizacija;nekonvencionalna sekrecija;ceramid fosfoetanolamin;vezava na nevretenčarske lipide;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-23
Letnik: ǂiss. ǂ12
Zvezek: ǂ[article] ǂ1973
Čas izdaje: Dec. 2020
DOI: 10.3390/microorganisms8121973
ID: 14354384