ǂa ǂfocus on nanomaterials
Craig Mayall (Avtor), Andraž Dolar (Avtor), Anita Jemec (Avtor), Sara Novak (Avtor), Jaka Razinger (Avtor), Francesco Barbero (Avtor), Victor Puntes (Avtor), Damjana Drobne (Avtor)

Povzetek

We compared the changes of selected immune parameters of Porcellio scaber to different stressors. The animals were either fed for two weeks with Au nanoparticles (NPs), CeO$_2$ NPs, or Au ions or body-injected with Au NPs, CeO$_2$ NPs, or lipopolysaccharide endotoxin. Contrary to expectations, the feeding experiment showed that both NPs caused a significant increase in the total haemocyte count (THC). In contrast, the ion-positive control resulted in a significantly decreased THC. Additionally, changes in phenoloxidase (PO)-like activity, haemocyte viability, and nitric oxide (NO) levels seemed to depend on the stressor. Injection experiments also showed stressor-dependant changes in measured parameters, such as CeO$_2$ NPs and lipopolysaccharide endotoxin (LPS), caused more significant responses than Au NPs. These results show that feeding and injection of NPs caused an immune response and that the response differed significantly, depending on the exposure route. We did not expect the response to ingested NPs, due to the low exposure concentrations (100 µg/g dry weight food) and a firm gut epithelia, along with a lack of phagocytosis in the digestive system, which would theoretically prevent NPs from crossing the biological barrier. It remains a challenge for future research to reveal what the physiological and ecological significance is for the organism to sense and respond, via the immune system, to ingested foreign material.

Ključne besede

imunski odziv;kopenski raki;nanodelci;spremembe imunskih parametrov;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 577
COBISS: 59056899 Povezava se bo odprla v novem oknu
ISSN: 2079-4991
Št. ogledov: 136
Št. prenosov: 43
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: imunski odziv;kopenski raki;nanodelci;spremembe imunskih parametrov;
Vrsta dela (COBISS): Članek v reviji
Strani: 1-18
Letnik: ǂ[iss.] ǂ4
Zvezek: ǂ[article] ǂ934
Čas izdaje: 6 Apr. 2021
DOI: 10.3390/nano11040934
ID: 14657172