magistrsko delo
Povzetek
V današnjem času je presnovni sindrom vse bolj pogosta presnovna motnja. Presnovni sindrom je zelo povezan s sladkorno boleznijo tipa 2 (SBT2) in duševnimi motnjami. Predvsem prevalenci anksioznosti in depresivnosti naraščata na globalni ravni. Namen raziskave je preučiti pojav anksioznemu in depresivnemu vedenju podobnega obnašanja na mišjem modelu SBT2. Pri tem nas je zanimal vpliv spola. V ta namen smo miši razdelili v tri skupine. Prva skupina je predstavljala kontrolno skupino, ki smo jo hranili s standardno prehrano (CD). Drugo skupino smo hranili z zahodno dieto (WD). Tretjo skupino smo po obdobju hranjenja z WD izpostavili kratkotrajni kalorični restrikciji (RCD), saj nas je zanimalo, ali bo kalorična restrikcija, ki povzroči remisijo SBT2, povzročila tudi zmanjšanje nagnjenosti k anskioznemu in depresivnemu vedenju podobnemu obnašanju. Vpliv prehranskega režima smo ocenjevali s pomočjo vedenjskih testov. Za ocenjevanje lokomotorike smo uporabili test odprtega polja (OFT), za ocenjevanje anskioznemu vedenju podobnega obnašanja test dvignjenega labirinta v obliki križa (EPM) in za ocenjevanje depresivnemu vedenju podobnega obnašanja test prisilnega plavanja (FST). Ugotovili smo, da indukcija SBT2 z zahodno dieto ni vplivala na pojav anskioznemu in depresivnemu vedenju podobnega obnašanja pri miših. Prav tako na pojav omenjenih motenj ni vplival spol. Ugotovili smo, da so bile WD samice bolj aktivne kot WD samci (bile so hitrejše, prehodile so večjo razdaljo in manj časa mirovale). V skladu s tem, da indukcija SBT2 ni vplivala na teste anskioznemu in depresivnemu podobnemu obnašanju, tudi kalorična restrikcija ni vplivala na k anskioznemu in depresivnemu vedenju podobnemu obnašanju. Opazili smo vpliv habituacije na nekatere vedenjske teste. Pri testu OFT smo opazili zmanjšanje raziskovalnega vedenja pri ponavljanju testa, pri testu EPM pa zmanjšano raziskovanje odprtih krakov pri ponavljanju testa.
Ključne besede
magistrska dela;presnovni sindrom;sladkorna bolezen;anksioznost;depresivnost;zahodna dieta;kalorična restrikcija;vedenjski testi;
Podatki
Jezik: |
Slovenski jezik |
Leto izida: |
2022 |
Tipologija: |
2.09 - Magistrsko delo |
Organizacija: |
UM FNM - Fakulteta za naravoslovje in matematiko |
Založnik: |
[N. Polšak] |
UDK: |
57.017.7:613.86:616.379-008.64(043.2) |
COBISS: |
103367939
|
Št. ogledov: |
194 |
Št. prenosov: |
14 |
Ocena: |
0 (0 glasov) |
Metapodatki: |
|
Ostali podatki
Sekundarni jezik: |
Angleški jezik |
Sekundarni naslov: |
Influence of metabolic syndrome on the development of anxiety and depressive behaviors on mouse model of type 2 diabetes mellitus |
Sekundarni povzetek: |
Nowadays, metabolic syndrome is an increasingly common metabolic disorder. Metabolic syndrome is strongly associated with type 2 diabetes (T2D) and mental disorders. In particular, the prevalence of anxiety and depression is growing globally. The aim of the study was to examine the occurrence of anxiety and depressive and depressive-like behaviors in the T2D mouse model. We were also interested in the influence of gender. For this purpose, we divided the mice into three groups. The first group represented a control group fed a classical diet (CD). The second group was fed a western diet (WD). The third group was exposed to a reduced-calorie diet (RCD) after a period of WD feeding, as we were interested in whether caloric restriction, which causes T2D remission, would also lead to a reduction in predisposition to anxious and depressive behaviors. The impact of the diet was assessed using behavioral tests. The Open Field Test (OFT) was used to assess locomotorization, the Elevated Plus Maze (EPM) test to assess anxious-like behavior, and the Forced Swimming Test (FST) to assess depressive-like behavior. We found that induction of T2D by the western diet did not affect the occurrence of anxious and depressive behaviors of similar behavior in mice. The occurrence of these disorders was also not influenced by gender. However, we found that WD females were more active than WD males (they were faster, walked farther distances, and spent less time at rest). Consistent with the fact that T2D induction did not affect tests for anxious and depressive-like behavior, caloric restriction did not affect anxious and depressive-like behavior. We observed the impact of habituation on some behavioral tests. In the OFT test, we observed a decrease in research behavior when repeating the test, and in the EPM test, a decrease in exploration of open arms of the labyrinth when repetitions of the test were observed. |
Sekundarne ključne besede: |
master theses;metabolic syndrome;diabetes;anxiety;depression;western diet;caloric restriction;behavioral tests;Sladkorna bolezen;Tesnoba;Depresija (psihologija);Dieta;Univerzitetna in visokošolska dela; |
Vrsta dela (COBISS): |
Magistrsko delo/naloga |
Komentar na gradivo: |
Univ. v Mariboru, Fak. za naravoslovje in matematiko, Oddelek za biologijo |
Strani: |
XI f., 55 str. |
ID: |
14725078 |