Funkcionalizacija zaščitnih mask z uporabo naravnih snovi

diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje

Maša Vračevič (Avtor), Marjana Simonič (Mentor), Lidija Fras Zemljič (Komentor), Olivija Plohl (Komentor)

Povzetek

Zaščitne maske filtrirajo mikrobne delce in ščitijo uporabnika pred škodljivimi delci. Ključnega pomena za zaščito uporabnika in ljudi v njegovi okolici je zaščita pred nalezljivimi boleznimi. V ta namen je potrebna uporaba protimikrobnih sredstev, kot sta laktoferina in katehin.Za lažji nanos na površino maske smo pripravili raztopini, ki smo ju s pršenjem nanesli na notranjo površino sloja maske iz polipropilena (PP). Prisotnost potencialnih antiviralnih agensov na površini smo določili z gravimetrično tehniko tehtanja vzorcev pred in po pršenju, s spektroskopskimi metodami in z merjenjem naboja na površini s tehniko določanja zeta potenciala. Pri metodi x-žarkovne rentgenske spektroskopije (XPS) smo določali elementno sestavo na podlagi fotoefekta. Prisotnost agensov smo potrdili, če je bil na površini prisoten element, ki ni izhajal iz polipropilena. Z metodo infrardeče spektroskopije s Fourierjevo transformacijo (ATR-FTIR) smo na podlagi sevanja infrardeče svetlobe v spektrometru dobili spektre. Vrhove spektrov laktoferina in katehina smo primerjali z dobljenimi spektri vzorcev in v primeru ujemanja vrhov določili prisotnost nanešenih agensov na površini. Da smo preverili kemijsko stabilnost vzorcev po simulaciji človeške sape, smo ponovno uporabili gravimetrično tehniko tehtanja in spektroskopsko metodo ATR-FTIR. Za določanje omočljivosti modificirane površine smo uporabili metodo goniometrije, kjer smo z doziranjem kapljic na površino izmerili stične kote. Zračno prepustnost vzorcev smo merili na podlagi različnih tlakov na napravi Karl Shroder KG air permeability tester. Protimikrobno delovanje površine vzorcev pa smo določili s testi antiviralnosti na petrijevkah. Analize, ki so bile narejene za dokaz prisotnosti slojev laktoferina in katehina na površini, so v večini pokazale prisotnost raztopine laktoferina. Sloj katehina ni bil prisoten na površini vzorca glede na rezultate merjenja zeta potenciala in spektrov dobljenih pri metodi ATR-FTIR. Prisotnost posameznih slojev nanosa pa je bila zaznana pri rezultatih antiviralnosti. Ti so pokazali, da v primeru popršenih vzorcev zunanjega sloja maske pride do redukcije oziroma zmanjšanja nastajanja modelnih virusov v primerjavi z nepopršenim materialom. Izmerjeni stični koti so pokazali, da imamo hidrofobno površino vzorcev, kar zagotavlja manjšo omočlljivost materiala. Z določanjem zračne prepustnosti smo želeli dokazati, da material omogoča zadostno prepustnost zraka, če na površino materiala nanesemo sloje antiviralnih agensov.

Ključne besede

polipropilen;laktoferin;katehin;antiviralnost;spektroskopija;diplomske naloge;

Podatki

Jezik:	Slovenski jezik
Leto izida:	2022
Tipologija:	2.11 - Diplomsko delo
Organizacija:	UM FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Založnik:	[M. Vračević]
UDK:	543.42:]604.4:615.281.8](043.2)
COBISS:	120514307
Št. ogledov:	57
Št. prenosov:	18
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Angleški jezik
Sekundarni naslov:	Functionalization of surgical masks with application of natural substances
Sekundarni povzetek:	Surgical masks filter out microbial particles and protect the user from harmful particles. However, the mask itself does not provide adequate protection against the transmission of micro-organisms, which is essential to protect the user and those around him/her from infectious diseases. This protection requires the use of antimicrobials such as lactoferrin and catechin. To facilitate the application on the mask surface, two solutions were prepared and sprayed on the inner surface of the mask layer made of polypropylene (PP). The presence of solutions on the surface was determined by gravimetric weighing of the samples before and after spraying, by spectroscopic methods and by measuring the charge on the surface using the zeta potential technique. The X-ray photoelectron spectroscopy (XPS) method was used to determine the elemental composition based on the photoeffect. The presence of agents was confirmed if an element was present on the surface that was not present on the polypropylene surface. The Fourier transform infrared spectroscopy (ATR-FTIR) method was used to obtain spectra based on the infrared radiation in the spectrometer. The peaks of the lactoferrin and catechin spectra were compared with the obtained spectra of the samples and, if the peaks matched, the presence of solutes on the surface was determined. To verify the chemical stability of the samples after simulated human breathing, the gravimetric weighing technique and the ATR-FTIR spectroscopic method were again used. To determine the wettability of the modified surface, we used goniometry, where we measured the contact angles by dispensing droplets onto the surface. The air permeability of the samples was measured at different pressures on a Karl Shroder KG air permeability tester. The antimicrobial activity of the surface of the samples was determined by antiviral tests on petri dishes. Analyses carried out to demonstrate the presence of lactoferrin and catechin layers on the surface showed the presence of lactoferrin solution in the majority of cases. The catechin layer was not present on the surface of the sample according to the results of the zeta potential measurements and spectra obtained by ATR-FTIR. However, the presence of individual coating layers was detected in the antivirality results. These showed that when the outer layer of the mask is sprayed, there is a reduction or decrease in the formation of model viruses compared to the unsprayed material. The measured contact angles showed that the samples have a hydrophobic surface, which ensures that the material is less wettable. By determining the air permeability, we wanted to demonstrate that the material provides sufficient air permeability when layers of antiviral agents are applied to the surface of the material.
Sekundarne ključne besede:	polypropylene;lactoferrin;catechin;antivirality;spectroscopy;
Vrsta dela (COBISS):	Diplomsko delo/naloga
Komentar na gradivo:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Strani:	1 spletni vir (1 datoteka PDF (VIII, 29 f.))
ID:	16191175