diplomsko delo univerzitetnega študijskega programa I. stopnje
Doroteja Golob (Avtor), Uroš Potočnik (Mentor), Tomaž Büdefeld (Komentor)

Povzetek

Akutna limfoblastna levkemija celic T (T-ALL) in limfoblastni limfom celic T (T-LBL) sta vrsti krvnih rakov, ki nastanejo iz timocitov v timusu. Pomemben dejavnik pri razvoju rakavih celic je uspešno spopadanje celice z oksidativnim stresom, ki ga izzovejo reaktivne kisikove spojine. Reaktivne kisikove spojine (RKS) so heterogena skupina majhnih molekul, ionov in radikalov, za katere je značilna visoka reaktivnost; oksidativni stres pa lahko opredelimo kot stanje znotraj celice, ki je rezultat prekomerne tvorbe RKS ob oviranih ali odsotnih mehanizmih, ki bi vzdrževali redoks homeostazo. Ker še vedno ni povsem jasno, ali sta T ALL in T-LBL ena bolezen z različnimi bolezenskimi znaki, ali predstavljata dve različni bolezni, ki prizadeneta limfocite T, smo se v diplomskem delu odločili proučiti morebitne razlike v celičnem odzivu celičnih linij T-ALL, Jurkat, in T-LBL, SUP-T1, na oksidativni stres. Celični odziv smo spremljali skozi kvantifikacijo živih in mrtvih celic, viabilnost, celični metabolizem ter s kvantifikacijo reaktivnih kisikovih spojin. Primerjava rasti celičnih linij Jurkat in SUP T1 v normoksičnih pogojih in v prisotnosti oksidativnega stresa, je pokazala na negativen vpliv oksidativnega stresa na rast celic, ni pa pokazala razlik med celičnima linijama Jurkat in SUP T1 v številu živih celic. Delež mrtvih celic se ni razlikoval niti med celičnima linijama Jurkat in SUP-T1, niti glede na izpostavljenost oksidativnemu stresu. Edina statistično značilna razlika se je pokazala v viabilnosti, kjer je izpostavljenost celic Jurkat oksidativnemu stresu privedla do znižane viabilnosti, le-ta je bila, 72 ur po nasaditvi, statistično značilno nižja, v primerjavi z viabilnostjo tretiranih celic SUP-T1 (F(3, 10) = 26.11, p < 0,001; post hoc: p < 0,05). Tudi rezultati metabolnega testa in testa za kvantifikacijo reaktivnih kisikovih spojin niso pokazali razlik med celičnima linijama Jurkat in SUP-T1. Naši rezultati torej kažejo na veliko podobnost celičnih linij Jurkat in SUP-T1, v spopadanju z oksidativnim stresom, in podpirajo trenutno klasifikacijo Svetovne zdravstvene organizacije WHO in Mednarodnega panela za proučevanje limfomov, ki smatra T-ALL in T-LBL kot eno bolezen z različnimi bolezenskimi znaki.

Ključne besede

akutna limfoblastna levkemija celic T;limfoblastni limfom celic T;reaktivne kisikove spojine;oksidativni stres;diplomske naloge;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.11 - Diplomsko delo
Organizacija: UM FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Založnik: [D. Golob]
UDK: 576.5:616.155.392(043.2)
COBISS: 132098051 Povezava se bo odprla v novem oknu
Št. ogledov: 87
Št. prenosov: 24
Ocena: 0 (0 glasov)
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Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Cellular response to hydrogen peroxide-induced oxidative stress in T-cell acute lymphoblastic leukemia and T-cell lymphoblastic lymphoma cell lines
Sekundarni povzetek: T-cell acute lymphoblastic leukaemia (T-ALL) and T-cell lymphoblastic lymphoma (T-LBL) are types of blood cancer that arise from thymocytes in the thymus. An important factor in the development of cancer cells is the successful coping of a cell with oxidative stress, induced by reactive oxygen species. Reactive oxygen species (ROS) are a heterogeneous group of small molecules, ions and radicals characterised by high reactivity, while oxidative stress can be defined as an intracellular state, resulting from excessive ROS production in the presence of impaired or absent mechanisms, which could maintain redox homeostasis. Since it is still unclear whether T-ALL and T-LBL are the same disease with different symptoms or represent two distinct diseases affecting T lymphocytes, we decided to study possible differences in the cellular response of T-ALL cell line, Jurkat, and T-LBL cell line, SUP-T1, to oxidative stress. The cellular response was monitored through quantification of live and dead cells, viability, cellular metabolism and by quantification of reactive oxygen species. Comparison of the growth of Jurkat and SUP-T1 cell lines under normoxic conditions and in the presence of oxidative stress, showed a negative effect of oxidative stress on cell growth, but there were no differences between Jurkat and SUP-T1 cell lines in the number of live cells. The proportion of dead cells did not differ between Jurkat and SUP-T1 cell lines, nor did it differ between cells under normoxic conditions and those in the presence of oxidative stress. Viability showed the only statistically significant difference, where exposure of Jurkat cells to oxidative stress, led to reduced viability, which was statistically significantly lower, compared to the viability of SUP-T1 cells exposed to oxidative stress (F(3, 10) = 26.11, p < 0,001; post hoc: p < 0,05). The results of the metabolic and reactive oxygen species quantification assays also showed no differences between Jurkat and SUP-T1 cell lines. Our results therefore indicate a high degree of similarity between Jurkat and SUP-T1 cell lines in the way they cope with oxidative stress and support the current classification, adopted by World Health Organization (WHO) and International Panel for the Study of Lymphomas, which considers T-ALL and T-LBL as the same disease with different symptoms.
Sekundarne ključne besede: T-cell acute lymphoblastic leukemia;T-cell lymphoblastic lymphoma;reactive oxygen species;oxidative stress;
Vrsta dela (COBISS): Diplomsko delo/naloga
Komentar na gradivo: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Strani: 1 spletni vir (1 datoteka PDF (VIII, 27 f.))
ID: 16310837