Maja Zabel (Avtor), Milena Weber (Avtor), Bernhard Kratzer (Avtor), Cordula Köhler (Avtor), Beatrice Jahn-Schmid (Avtor), Gabriele Gadermaier (Avtor), Pia Gattinger (Avtor), Urška Bidovec (Avtor), Peter Korošec (Avtor), Ursula Smole (Avtor), Rudolf Valenta (Avtor), Winfried F. Pickl (Avtor)

Povzetek

Background. Worldwide, pollen of the weed mugwort (Artemisia vulgaris) is a major cause of severe respiratory allergy, with its major allergen, Art v 1, being the key pathogenic molecule for millions of patients. Humanized mice transgenic for a human T-cell receptor specific for the major Art v 1 T-cell epitope and the corresponding HLA have been made. Objective. We sought to characterize IgE epitopes of Art v 1–sensitized patients and humanized mice for molecular immunotherapy of mugwort allergy. Methods. Four overlapping peptides incorporating surface-exposed amino acids representing the full-length Art v 1 sequence were synthesized and used to search for IgE reactivity to sequential epitopes. For indirect mapping, peptide-specific rabbit antibodies were raised to block IgE against surface-exposed epitopes on folded Art v 1. IgE reactivity and basophil activation studies were performed in clinically defined mugwort-allergic patients. Secondary structure of recombinant (r) Art v 1 and peptides was determined by circular dichroism spectroscopy. Results. Mugwort-allergic patients and humanized mice sensitized by allergen inhalation showed IgE reactivity and/or basophil activation mainly to folded, complete Art v 1 but not to unfolded, sequential peptide epitopes. Blocking of allergic patients’ IgE with peptide-specific rabbit antisera identified a hitherto unknown major conformational IgE binding site in the C-terminal Art v 1 domain. Conclusions. Identification of the new major conformational IgE binding site on Art v 1, which can be blocked with IgG raised against non-IgE reactive Art v 1 peptides, is an important basis for the development of a hypoallergenic peptide vaccine for mugwort allergy.

Ključne besede

alergija za pelod pelina;epitop IgE;specifična imunoterapija;mugwort pollen allergy;IgE epitope;allergen-specific immunotherapy;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
UDK: 616-097
COBISS: 116269571 Povezava se bo odprla v novem oknu
ISSN: 1097-6825
Št. ogledov: 9
Št. prenosov: 2
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: Allergy and immunology;Hypersensitivity;Allergens;Epitope mapping;Artemisia;Desensitizaion, immunologic;Preobčutljivost;Alergeni;Alergija in imunologija;Kartiranje epitopov;Imunološka desenzibilizacija;
Komentar vira: Soavtorja iz Slovenije: Urška BIdovec-Srojković, Peter Korošec; Nasl. z nasl. zaslona; Opis vira z dne 22. 7. 2022;
Strani: str. 1-11
Letnik: ǂVol. ǂ150
Zvezek: ǂiss. ǂ
Čas izdaje: 2022
DOI: 10.1016/j.jaci.2022.04.031
ID: 16327083