magistrsko delo
Tjaša Damijan (Avtor), Tomaž Langerholc (Mentor), Maja Šikić Pogačar (Komentor), Anja Klančnik (Komentor)

Povzetek

Mikroaerofilne bakterije Campylobacter jejuni so kljub zahtevnim pogojem rasti zelo razširjeni mikroorganizmi in najpogostejši povzročitelji akutnega bakterijskega gastroenteritisa pri ljudeh po vsem svetu. Predpostavlja se, da ima pri preživetju v naravnem okolju pomembno vlogo tvorba biofilma. Prvi in ključni korak, potreben za uspešno tvorbo biofilma, je adhezija bakterij na ustrezno površino. V magistrskem delu je bila ovrednotena sposobnost adhezije in invazije bakterij Campylobacter jejuni na/v humane črevesne celice karcinogenega izvora Caco-2. V raziskavo so bili vključeni referenčni sev Campylobacter jejuni (divji sev NCTC 11168) in njegovi mutanti z mutacijami v genih chuA, csrA, kpsM, luxS, omp50, rrpA, rrpB, trpE in Cj1191c. Vpliv mutacij v izbranih genih na sposobnost adhezije in invazije bakterij Campylobacter jejuni je bil ovrednoten z metodo štetja kolonij (CFU/ml). Pred tem je bil ovrednoten vpliv mutantov na metabolno aktivnost celic Caco-2 s kolorimetričnim testom z uporabo reagenta MTT. Statistična analiza je pokazala, da so mutacije v genih chuA, csrA, luxS, omp50 in rrpB statistično značilno zmanjšale sposobnost adhezije bakterij Campylobacter jejuni na celice Caco-2. Mutacije v genih kpsM, trpE in Cj1191c so statistično značilno povečale sposobnost adhezije bakterij Campylobacter jejuni na celice Caco-2. Sposobnost invazije v celice Caco-2 sta imela samo mutanta z mutacijo v genu rrpB in genu luxS. Ostali mutanti niso invadirali v celice Caco-2. Potrebne so nadaljnje raziskave za razvoj strategij, ki omejujejo število okužb in omogočajo nadzor bakterij v živilih

Ključne besede

Campylobacter jejuni;tvorba biofilma;adhezija;invazija;celice Caco-2;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.09 - Magistrsko delo
Organizacija: UM FKBV - Fakulteta za kmetijstvo in biosistemske vede
Založnik: [T. Damijan]
UDK: 579:579.8:628.353.153(043)=163.6
COBISS: 136101891 Povezava se bo odprla v novem oknu
Št. ogledov: 31
Št. prenosov: 2
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: The influence of biofilm-relevant gene mutations on the adhesion and invasion ability of campylobacter jejuni in caco-2 cells
Sekundarni povzetek: Despite its difficult growth conditions, the microaerophilic bacterium Campylobacter jejuni is a widespread microorganism and the most common cause of acute bacterial gastroenteritis in humans worldwide. Biofilm formation is thought to play an important role in its survival in natural environments. The first and most important step for the successful formation of a biofilm is the adhesion of bacteria to a suitable surface. In this master thesis, the adhesion and invasion ability of Campylobacter jejuni bacteria on/in carcinogenic human intestinal cells Caco-2 was evaluated. The reference strain Campylobacter jejuni (wild strain NCTC 11168) and its mutants with mutations in the genes chuA, csrA, kpsM, luxS, omp50, rrpA, rrpB, trpE and Cj1191c were included in the study. The effects of mutations in selected genes on the adhesion and invasion ability of Campylobacter jejuni were evaluated using the colony count method (CFU/ml). First, the influence of the mutations on the metabolic activity of Caco-2 cells was assessed using a colorimetric MTT assay. Mutations in the genes chuA, csrA, luxS, omp50 and rrpB statistically significantly decreased the adhesiveness of Campylobacter jejuni bacteria to Caco-2 cells. In contrast, mutations in the kpsM, trpE and Cj1191c genes statistically significantly increased the adhesion ability of Campylobacter jejuni bacteria to Caco-2 cells. Only one mutant with a mutation in the rrpB gene and one mutant with a mutation in the luxS gene had invasive abilities in Caco-2 cells. The other mutants did not invade Caco-2 cells. Further research is needed to develop strategies to limit the number of infections and control bacteria in food
Sekundarne ključne besede: Campylobacter jejuni;biofilm formation;adhesion;invasion;Caco-2 cells;Univerzitetna in visokošolska dela;
Vrsta dela (COBISS): Magistrsko delo/naloga
Komentar na gradivo: Univ. v Mariboru, Fak. za kmetijstvo in biosistemske vede
Strani: 1 spletni vir (1 datoteka PDF (XII, 79 str.))
ID: 17228003