Vasyl Shvalya (Avtor), Martina Modic (Avtor), Cene Skubic (Avtor), Nejc Nadižar (Avtor), Janez Zavašnik (Avtor), Damjan Vengust (Avtor), Aleksander Zidanšek (Avtor), Damjana Rozman (Avtor), Uroš Cvelbar (Avtor)

Povzetek

It is shown that surface-enhanced Raman spectroscopy (SERS) can identify bacteria based on their genomic DNA composition, acting as a “sample-distinguishing marker”. Successful spectral differentiation of bacterial species was accomplished with nanogold aggregates synthesized through single-step plasma reduction of the ionic gold-containing vapored precursor. A high enhancement factor (EF = 10$^7$) in truncated coupled plasmonic particulates allowed SERS-probing at nanogram sample quantities. Simulations confirmed the occurrence of the strongest electric field confinement within nanometric gaps between gold dimers/chains from where the molecular fingerprints of bacterial DNA fragments gained photon scattering enhancement. The most prominent Raman modes linked to fundamental base-pair molecular vibrations were deconvoluted and used to proceed with nitrogenous base content estimation. The genomic composition (percentage of guanine-cytosine and adenine-thymine) was successfully validated by third-generation sequencing using nanopore technology, further proving that the SERS technique can be employed to swiftly specify bioentities by the discriminative principal-component statistical approach.

Ključne besede

DNA genomic ration;plasma electrochemical reduction;coupled plasmonic nanogold;DNA Raman fingerprints;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL MF - Medicinska fakulteta
UDK: 533
COBISS: 127669763 Povezava se bo odprla v novem oknu
ISSN: 1530-6992
Št. ogledov: 99
Št. prenosov: 20
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Vrsta dela (COBISS): Članek v reviji
Strani: str. 9757-9765
Letnik: ǂVol. ǂ22
Zvezek: ǂiss. ǂ23
Čas izdaje: Dec. 2022
DOI: 10.1021/acs.nanolett.2c02835
ID: 18003274