Michaela Hympanova (Avtor), Saša Terlep (Avtor), Aneta Markova (Avtor), Lukas Prchal (Avtor), Iztok Dogša (Avtor), Lenka Pulkrabkova (Avtor), Marketa Benkova (Avtor), Jan Marek (Avtor), David Stopar (Avtor)

Povzetek

An increasing microbial resistance to known antibiotics raises a demand for new antimicrobials. In this study the antimicrobial properties of a series of new N-Alkylpyridinium quaternary ammonium compounds (QACs) with varying alkyl chain lengths were evaluated for several nosocomial pathogens. The chemical identities of the new QACs were determined by NMR, LC-MS, and HRMS. All the planktonic bacteria tested were susceptible to the new QACs as evaluated by MIC and MBC assays. The antimicrobial effect was most pronounced against Staphylococcus aureus clinical isolates. Live/dead staining CLSM was used to test the effectiveness of the QACs in biofilms. The effectiveness was up to 10-fold lower than in the plankton. When QACs were used as irrigants in Er:YAG – SSP photoacoustic steaming, their effectiveness significantly increased. The combined use of irrigants and photoacoustic streaming increased biofilm removal from the surface and increased the killing rate of the cells remaining on the surface. This may allow for a shorter chemical exposure time and lower dosage of QACs used in applications. The results demonstrate that the new QACs have potential to be applied as antibacterial compounds effective against planktonic and biofilm bacteria as well as irrigants in removal of difficult-to-reach biofilms.

Ključne besede

biofilmi;bakterijski biofilmi;Staphylococcus aureus;kvarterne amonijeve spojine;protimikrobna aktivnost;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 579.6
COBISS: 33768963 Povezava se bo odprla v novem oknu
ISSN: 1664-302X
Št. ogledov: 24
Št. prenosov: 9
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: biofilmi;bakterijski biofilmi;Staphylococcus aureus;kvarterne amonijeve spojine;protimikrobna aktivnost;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-12, article 573951
Zvezek: ǂVol. ǂ11
Čas izdaje: Oct. 2020
DOI: 10.3389/fmicb.2020.573951
ID: 18177097