Tina Smolič (Avtor), Petra Tavčar Verdev (Avtor), Anemari Horvat (Avtor), Urška Černe (Avtor), Ana Halužan Vasle (Avtor), Larisa Tratnjek (Avtor), Mateja Erdani-Kreft (Avtor), Nicole Scholz (Avtor), Maja Matis (Avtor), Toni Petan (Avtor), Robert Zorec (Avtor), Nina Vardjan (Avtor)

Povzetek

When the brain is in a pathological state, the content of lipid droplets (LDs), the lipid storage organelles, is increased, particularly in glial cells, but rarely in neurons. The biology and mechanisms leading to LD accumulation in astrocytes, glial cells with key homeostatic functions, are poorly understood. We imaged fluorescently labeled LDs by microscopy in isolated and brain tissue rat astrocytes and in glia-like cells in Drosophila brain to determine the (sub)cellular localization, mobility, and content of LDs under various stress conditions characteristic for brain pathologies. LDs exhibited confined mobility proximal to mitochondria and endoplasmic reticulum that was attenuated by metabolic stress and by increased intracellular Ca2+, likely to enhance the LD-organelle interaction imaged by electron microscopy. When de novo biogenesis of LDs was attenuated by inhibition of DGAT1 and DGAT2 enzymes, the astrocyte cell number was reduced by ~40%, suggesting that in astrocytes LD turnover is important for cell survival and/or proliferative cycle. Exposure to noradrenaline, a brain stress response system neuromodulator, and metabolic and hypoxic stress strongly facilitated LD accumulation in astrocytes. The observed response of stressed astrocytes may be viewed as a support for energy provision, but also to be neuroprotective against the stress-induced lipotoxicity.

Ključne besede

astrocytes;stress stimuli;metabolism;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL MF - Medicinska fakulteta
UDK: 616-092
COBISS: 52537347 Povezava se bo odprla v novem oknu
ISSN: 0894-1491
Št. ogledov: 24
Št. prenosov: 3
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: astrociti;stresni dražljaji;presnova;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1540-1562
Letnik: ǂVol. ǂ69
Zvezek: ǂiss. ǂ6
Čas izdaje: 2021
DOI: 10.1002/glia.23978
ID: 18220004