Marina Dermastia (Avtor), Špela Tomaž (Avtor), Rebeka Strah (Avtor), Tjaša Lukan (Avtor), Anna Coll Rius (Avtor), Barbara Dušak (Avtor), Timotej Čepin (Avtor), Aleš Kladnik (Avtor), Maja Zagorščak (Avtor), Kristina Gruden (Avtor), Maruša Pompe Novak (Avtor)

Povzetek

The pathogenicity of intracellular plant pathogenic bacteria is associated with the action of pathogenicity factors/effectors, but their physiological roles for most phytoplasma species, including ‘Candidiatus Phytoplasma solani’ are unknown. Six putative pathogenicity factors/effectors from six different strains of ‘Ca. P. solani’ were selected by bioinformatic analysis. The way in which they manipulate the host cellular machinery was elucidated by analyzing Nicotiana benthamiana leaves after Agrobacterium-mediated transient transformation with the pathogenicity factor/effector constructs using confocal microscopy, pull-down, and co-immunoprecipitation, and enzyme assays. Candidate pathogenicity factors/effectors were shown to modulate plant carbohydrate metabolism and the ascorbate–glutathione cycle and to induce autophagosomes. PoStoSP06, PoStoSP13, and PoStoSP28 were localized in the nucleus and cytosol. The most active effector in the processes studied was PoStoSP06. PoStoSP18 was associated with an increase in phosphoglucomutase activity, whereas PoStoSP28, previously annotated as an antigenic membrane protein StAMP, specifically interacted with phosphoglucomutase. PoStoSP04 induced only the ascorbate–glutathione cycle along with other pathogenicity factors/effectors. Candidate pathogenicity factors/effectors were involved in reprogramming host carbohydrate metabolism in favor of phytoplasma own growth and infection. They were specifically associated with three distinct metabolic pathways leading to fructose-6-phosphate as an input substrate for glycolysis. The possible significance of autophagosome induction by PoStoSP28 is discussed.

Ključne besede

autophagosome;effector;glycolysis;pathogenicity factor;StAMP;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
Založnik: Frontiers Media
UDK: 632
COBISS: 161925891 Povezava se bo odprla v novem oknu
ISSN: 1664-462X
Št. ogledov: 8
Št. prenosov: 5
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni naslov: ‘Ca. P. solani’ pathogenicity factors/effectors modulation of host plant metabolism
Sekundarne ključne besede: Candidatus Phytoplasma solani;
Komentar vira: Soavtorji: Špela Tomaž, Rebeka Strah, Tjaša Lukan, Anna Coll, Barbara Dušak, Barbara Anžič, Timotej Čepin, Stefanie Wienkoop, Aleš Kladnik, Maja Zagorščak, Monika Riedle-Bauer, Christina Schönhuber, Wolfram Weckwerth, Kristina Gruden, Thomas Roitsch, Maruša Pompe Novak and Günter Brader; Nasl. z nasl. zaslona; Opis vira z dne 22. 8. 2023;
Strani: str. 1-16
Letnik: ǂVol. ǂ14
Zvezek: ǂ[art.] ǂ1232367
Čas izdaje: 2023
DOI: 10.3389/fpls.2023.1232367
ID: 19841911