Spektrofotometrično merjenje fluorescence, fosforescence in sipanja

diplomsko delo

Lucija Švent (Avtor), Aleš Fajmut (Mentor)

Povzetek

V diplomskem delu obravnavamo energijska stanja v atomih in molekulah, lastnosti fluorescence, fosforescence in sipanja kot prehode med molekulskimi energijskimi stanji. Opišemo tudi Beer-Lambertov zakon za vse tri pojave. Meritve opravimo s spektrofotometrom MUT Tristan Light in s preoblikovano transmisijsko komoro. Oprema je bila nabavljena v okviru Norveškega finančnega mehanizma. Z meritvami pokažemo Stokesov premik za fluorescenco, spektralni maksimum za fosforescenco in odvisnost intenzitete svetlobe od valovne dolžine. Z meritvami tudi pokažemo, da je prek Beer-Lambertovega zakona mogoče izračunati neznano koncentracijo raztopine tudi iz gostot fluoresciranega oziroma sipanega svetlobnega toka vzorcev. Del diplomskega dela je namenjen zgodovini raziskovanj pojavov in njihovim aplikacijam v moderni znanosti.

Ključne besede

fizika;spektrofotometer;fluorescenca;fosforescenca;sipanje;absorpcija;energijski nivoji;diplomska dela;

Podatki

Jezik:	Slovenski jezik
Leto izida:	2012
Izvor:	Maribor
Tipologija:	2.11 - Diplomsko delo
Organizacija:	UM FNM - Fakulteta za naravoslovje in matematiko
Založnik:	[L. Švent]
UDK:	53(043.2)
COBISS:	19132168
Št. ogledov:	2282
Št. prenosov:	506
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Angleški jezik
Sekundarni naslov:	SPECTROPHOTOMETRIC MEASUREMENT OF FLUORESCENCE, PHOSPHORESCENCE AND SCATTERING
Sekundarni povzetek:	In the thesis we study and describe the energy levels of atoms and molecules, possible transitions between them, properties of fluorescence, phosphorescence and scattering from the point of view of transitions between molecular energy states. We also present the original form of Beer-Lambert's law as well as its modifications and applications in all three studied phenomena. The measurements were performed with the spectrometer MUT Tristan Light and the transformed transmission chamber. The equipment was acquired within the project financed from the Norway Grants. With the spectrophotometer we can measure the Stoke's fluorescence shift, phosphorescent spectral maximum and the dependence of the intensity of the scattered light with respect to their wavelengths. From the measured spectra of fluoresced or scattered light we were able to determine the concentrations of either fluorescent dyes or small particles in the liquid samples with the use of the modified Beer-Lambert's law. A part of the thesis is dedicated also to the presentation of a historical overview of these phenomena as well as of their applications in modern science.
Sekundarne ključne besede:	spectrophotometer;fluorescence;phosphorescence;scattering;absorption;light;Beer- Lambert's law;energy levels;
URN:	URN:SI:UM:
Vrsta dela (COBISS):	Diplomsko delo
Komentar na gradivo:	Univ. v Mariboru, Fak. za naravoslovje in matematiko, Oddelek za fiziko
Strani:	VII, 59 f.
Ključne besede (UDK):	mathematics;natural sciences;naravoslovne vede;matematika;physics;fizika;
ID:	19928