Genomic insights into the safety and bacteriocinogenic potential of isolates from artisanal fermented milk Matsoni

Vita Rozman (Avtor), Petra Mohar Lorbeg (Avtor), Nina Chanishvili (Avtor), Tomaž Accetto (Avtor), Elene Kakabadze (Avtor), Nino Grdzelishvili (Avtor), Maja Rupnik (Avtor), Bojana Bogovič Matijašić (Avtor)

Povzetek

Matsoni is a fermented milk common in the Caucasian region. In this study, whole-genome sequencing was performed with the aim of determining the taxonomic position of 25 previously selected candidate Matsoni starter cultures, identifying bacteriocin genes and prophages in their genomes, and evaluating their safety. In silico prediction of antibiotic resistance genes, virulence factors, bacteriocin genes and prophages was performed using dedicated databases and bioinformatics tools. With the exception of Enterococcus faecalis, which contained the mobile genetic element Tn916 with tetM and virulence factors, and Streptococcus thermophilus with mefE, the isolates were found to be safe for use as starter cultures. Thermophilin A, thermophilin 13, streptide and Blp genes were predicted in isolates of Streptococcus thermophilus, and genes for enterocins A, B, and X were found in Enterococcus faecium, but inhibition between strains was not observed. Several complete prophages were also detected in the genomes of Lactobacillus delbrueckii ssp. lactis. Lacticaseibacillus rhamnosus, Limosilactobacillus fermentum, Streptococcus thermophilus, Enterococcus faecalis, and Enterococcus faecium. Some of these isolates are good candidates for customised autochthonous Matsoni starter cultures which are an alternative to the commercial starter cultures currently used in the industry.

Ključne besede

mlečni izdelki;fermentirano mleko;Matsoni;genetika;genom;sekvenciranje;bakteriocini;profagi;

Podatki

Jezik:	Angleški jezik
Leto izida:	2023
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UL BF - Biotehniška fakulteta
UDK:	637.1:575
COBISS:	162265603
ISSN:	1096-1127
Št. ogledov:	65
Št. prenosov:	9
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	mlečni izdelki;fermentirano mleko;matsoni;genetika;genom;sekvenciranje;bakteriocini;profagi;
Vrsta dela (COBISS):	Članek v reviji
Strani:	str. 1-13
Letnik:	ǂVol. ǂ185
Zvezek:	[article no.] 115183
Čas izdaje:	2023
DOI:	10.1016/j.lwt.2023.115183
ID:	20959976