Surfactin molecules with a cone-like structure promote the formation of membrane domains with negative spontaneous curvature and induce membrane invaginations
Povzetek
Surfactin uniquely influences lipid bilayer structure by initially inducing membrane invaginations before solubi-lization. In this study, we exposed DOPC giant vesicles to various surfactin concentrations at different tempera-tures and observed surfactin-induced membrane invaginations by using differential interference contrast andconfocal laser fluorescence microscopy. These invaginations were stable at room temperature but not at highertemperatures. Surfactin molecules induce membrane nanodomains with negative spontaneous curvature andmembrane invaginations despite their intrinsic conical shape and intrinsic positive curvature. Considering theexperimentally observed capacity of surfactin to fluidize lipid acyl chains and induce partial dehydration of lipidheadgroups, we propose that the resulting surfactin-lipid complexes exhibit a net negative spontaneous curva-ture. We further conducted 3D numerical Monte Carlo (MC) simulations to investigate the behaviour of vesiclescontaining negative curvature nanodomains within their membrane at varying temperatures. MC simulationsdemonstrated strong agreement with experimental results, revealing that invaginations are preferentially formedat low temperatures, while being less pronounced at elevated temperatures. Our findings go beyond the expecta-tions of the Israelachvili molecular shape and packing concepts analysis. These concepts do not take into accountthe influence of specific interactions between neighboring molecules on the inherent shapes of molecules andtheir arrangement within curved membrane nanodomains. Our work contributes to a more comprehensive un-derstanding of the complex factors governing vesicle morphology and membrane organization and provides in-sight into the role of detergent-lipid interactions in modulating vesicle morphology.
Ključne besede
lipidne membrane;surfaktin;surfactin;lipid membranes;solubilization dynamics;curved membrane nanodomains;molecular shape;
Podatki
| Jezik: | Angleški jezik |
|---|---|
| Leto izida: | 2023 |
| Tipologija: | 1.01 - Izvirni znanstveni članek |
| Organizacija: | UL FE - Fakulteta za elektrotehniko |
| UDK: | 577.352 |
| COBISS: |
159013379
|
| ISSN: | 0021-9797 |
| Št. ogledov: | 250 |
| Št. prenosov: | 24 |
| Ocena: | 0 (0 glasov) |
| Metapodatki: |
|
Ostali podatki
| Sekundarni jezik: | Slovenski jezik |
|---|---|
| Sekundarne ključne besede: | lipidne membrane;surfaktin; |
| Vrsta dela (COBISS): | Članek v reviji |
| Strani: | str. 1193-1200 |
| Letnik: | ǂVol. ǂ650 |
| Zvezek: | ǂPart ǂB |
| Čas izdaje: | Nov. 2023 |
| DOI: | 10.1016/j.jcis.2023.07.057 |
| ID: | 21708468 |
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