Automated assessment of nanoparticle influence on giant lipid vesicles from video microscopy for bio-nano interaction studies

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Jezik:	Angleški jezik
Leto izida:	2011
Tipologija:	2.08 - Doktorska disertacija
Organizacija:	UL FRI - Fakulteta za računalništvo in informatiko
Založnik:	[J. Zupanc]
UDK:	004.932:66.017-022.532:577.115(043.3)
COBISS:	8500308
Št. ogledov:	53
Št. prenosov:	8
Ocena:	0 (0 glasov)
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Sekundarni jezik:	Slovenski jezik
Sekundarni naslov:	Avtomatizirano ugotavljanje vpliva nanodelcev na orjaške lipidne vezikle iz mikroskopskih posnetkov za študij bio-nano interakcij
Sekundarni povzetek:	Much evidence suggests that nanoparticles affect cell membrane stability and subsequently exert toxic effects. To assess these interactions research is often conducted on lipid vesicles as substitutes for cells. In this paper, we present a methodology which enables observing thousands of lipid vesicles and analyzing their shape transformations with the use of computerized image processing and data analysis methods. The aim of our study was to test whether this approach is appropriate for assessing effects of nanoparticles (C60 and CoFe2O4) on POPC lipid vesicles. In the experiments presented here, we show that nanoparticles provoked bursting of vesicles as well as their size and morphological changes. The novelty of our approach is in the possibility to investigate a large population of vesicles, and generate statistically relevant results. Our data demonstrates that nanoparticles affected lipid membranes and may have a potential to affect cell membranes as well.
Sekundarne ključne besede:	segmentacija slik;lipidni vezikli;video mikroskopija;mozaik;nanodelci;nanotoksikologija;mikroskopija večjih površin;virtualna mikroskopija;računalništvo;disertacije;
Vrsta datoteke:	application/pdf
Vrsta dela (COBISS):	Doktorska disertacija
Komentar na gradivo:	Univ. v Ljubljani, Fak. za računalništvo in informatiko
Strani:	XIII, 102 str.
ID:	23984459