Sandra Postić (Avtor), Marko Gosak (Avtor), Wen-Hao Tsai (Avtor), Johannes Pfabe (Avtor), Srdjan Sarikas (Avtor), Andraž Stožer (Avtor), Dean Korošak (Avtor), Shi-Bing Yang (Avtor), Marjan Rupnik (Avtor)

Povzetek

Extracellular pH has the potential to affect various aspects of the pancreatic beta cell function. To explain this effect, a number of mechanisms was proposed involving both extracellular and intracellular targets and pathways. Here, we focus on reassessing the influence of extracellular pH on glucose-dependent beta cell activation and collective activity in physiological conditions. To this end we employed mouse pancreatic tissue slices to perform high-temporally resolved functional imaging of cytosolic Ca2+ oscillations. We investigated the effect of either physiological H+ excess or depletion on the activation properties as well as on the collective activity of beta cell in an islet. Our results indicate that lowered pH invokes activation of a subset of beta cells in substimulatory glucose concentrations, enhances the average activity of beta cells, and alters the beta cell network properties in an islet. The enhanced average activity of beta cells was determined indirectly utilizing cytosolic Ca2+ imaging, while direct measuring of insulin secretion confirmed that this enhanced activity is accompanied by a higher insulin release. Furthermore, reduced functional connectivity and higher functional segregation at lower pH, both signs of a reduced intercellular communication, do not necessary result in an impaired insulin release.

Ključne besede

izločanje inzulina;razdražljivost membrane;kalijevi kanali;omrežje beta celic;kolektivna dejavnost;kalcijevi valovi;insulin secretion;membrane excitability;potassium channels;beta cell network;collective activity;calcium waves;pancreatic islets;pH-dependence;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM FGPA - Fakulteta za gradbeništvo, prometno inženirstvo in arhitekturo
Založnik: Frontiers Research Foundation
UDK: 616.8:577
COBISS: 117435907 Povezava se bo odprla v novem oknu
ISSN: 1664-2392
Št. ogledov: 27
Št. prenosov: 0
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: izločanje inzulina;razdražljivost membrane;kalijevi kanali;omrežje beta celic;kolektivna dejavnost;kalcijevi valovi;
Vrsta dela (COBISS): Znanstveno delo
Strani: str. 1-11
Zvezek: ǂVol. ǂ13
Čas izdaje: Jun. 2022
DOI: 10.3389/fendo.2022.916688
ID: 24373193