Martina Štampar (Avtor), Tim Ravnjak (Avtor), Ana-Marija Domijan (Avtor), Bojana Žegura (Avtor)

Povzetek

Bisphenol A (BPA) is one of the most commonly used substances in the manufacture ofvarious everyday products. Growing concerns about its hazardous properties, including endocrinedisruption and genotoxicity, have led to its gradual replacement by presumably safer analogues inmanufacturing plastics. The widespread use of BPA and, more recently, its analogues has increasedtheir residues in the environment. However, our knowledge of their toxicological profiles is limitedand their combined effects are unknown. In the present study, we investigated the toxic effectscaused by single bisphenols and by the combined exposure of BPA and its two analogues, BPAP andBPC, after short (24-h) and prolonged (96-h) exposure in HepG2 spheroids. The results showed thatBPA did not reduce cell viability in HepG2 spheroids after 24-h exposure. In contrast, BPAP andBPC affected cell viability in HepG2 spheroids. Both binary mixtures (BPA/BPAP and BPA/BPC)decreased cell viability in a dose-dependent manner, but the significant difference was only observedfor the combination of BPA/BPC (both at 40μM). After 96-h exposure, none of the BPs studiedaffected cell viability in HepG2 spheroids. Only the combination of BPA/BPAP decreased cellviability in a dose-dependent manner that was significant for the combination of 4μM BPA and 4μMBPAP. None of the BPs and their binary mixtures studied affected the surface area and growth ofspheroids as measured by planimetry. In addition, all BPs and their binary mixtures studied triggeredoxidative stress, as measured by the production of reactive oxygen species and malondialdehyde,at both exposure times. Overall, the results suggest that it is important to study the effects of BPsas single compounds. It is even more important to study the effects of combined exposures, as thecombined effects may differ from those induced by single compounds.

Ključne besede

BP analogues;hepatic in vitro 3D cell model;combined exposure;viability;oxidative stress;toxicology;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 577
COBISS: 148108803 Povezava se bo odprla v novem oknu
ISSN: 1420-3049
Št. ogledov: 17
Št. prenosov: 7
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: bisfenol A;bisfenol analogi;sferoidi;kombinirana izpostavitev;oksidativen stres;toksičnost;toksikologija;
Komentar vira: Nasl. z nasl. zaslona; Opis vira z dne 6. 4. 2023; Soavtorji: Tim Ravnjak, Ana-Marija Domijan and Bojana Žegura; Št. članka: 3085;
Strani: str. [1]-16
Letnik: ǂiss. ǂ7
Zvezek: ǂart. ǂ3085
Čas izdaje: 2023
DOI: 10.3390/molecules28073085
ID: 24512627