Tina Arnšek (Avtor), Nuša Golob (Avtor), Nastja Marondini (Avtor), Maruša Pompe Novak (Avtor), Kristina Gruden (Avtor), Tjaša Lukan (Avtor)

Povzetek

Hypersensitive response (HR)-conferred resistance is an effective defense response that can be determined by the N resistance genes. HR is manifested as the formation of cell death zones on inoculated leaves. Here, a protocol for studying the rate of cell death initiation by imaging inoculated leaves in the time between the cell death initiation and the cell death appearance using a digital microscope is presented. The digital microscope enables a continuous imaging process in desired intervals, which allows an accurate determination of cell death initiation rate up to minutes exactly, as opposed to hours in traditional methods. Imaging with the digital microscope is also independent of light and can therefore be used during day and night without disturbing the circadian rhythm of the plant. Different pathosystems resulting in programmed cell death development could be studied using this protocol with minor modifications. Overall, the protocol thus allows simple, accurate, and inexpensive identification of cell death initiation rate.

Ključne besede

digitalni mikroskop;celična smrt;inokulirani listi;digital microscope;cell death;inoculated leaves;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 577.2
COBISS: 177346819 Povezava se bo odprla v novem oknu
ISSN: 1940-087X
Št. ogledov: 26
Št. prenosov: 16
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: digitalni mikroskop;celična smrt;inokulirani listi;
Komentar vira: Nasl. z nasl. zaslona; Opis vira z dne 13. 12. 2023;
Strani: str. 1-9
Letnik: ǂVol. ǂ201
Zvezek: ǂ[article no.] ǂ3027
Čas izdaje: 2023
DOI: 10.3791/65824
ID: 24512667