Špela Tomaž (Avtor), Marko Petek (Avtor), Tjaša Lukan (Avtor), Karmen Pogačar (Avtor), Katja Stare (Avtor), Erica Teixeira Prates (Avtor), Aleksandra Usenik (Avtor), Dušan Turk (Avtor), Anna Coll Rius (Avtor), Kristina Gruden (Avtor), Daniel A. Jacobson (Avtor), Jan Zrimec (Avtor), Gregor Bajc (Avtor), Matej Butala (Avtor), Maruša Pompe Novak (Avtor), Ajda Taler-Verčič (Avtor)

Povzetek

TGA (TGACG-binding) transcription factors, which bind their target DNA through a conserved basic region leucine zipper (bZIP) domain, are vital regulators of gene expression in salicylic acid (SA)-mediated plant immunity. Here, we investigated the role of StTGA2.1, a potato (Solanum tuberosum) TGA lacking the full bZIP, which we named a mini-TGA. Such truncated proteins have been widely assigned as loss-of-function mutants. We, however, confirmed that StTGA2.1 overexpression compensates for SA-deficiency, indicating a distinct mechanism of action compared with model plant species. To understand the underlying mechanisms, we showed that StTGA2.1 can physically interact with StTGA2.2 and StTGA2.3, while its interaction with DNA was not detected. We investigated the changes in transcriptional regulation due to StTGA2.1 overexpression, identifying direct and indirect target genes. Using in planta transactivation assays, we confirmed that StTGA2.1 interacts with StTGA2.3 to activate StPRX07, a member of class III peroxidases (StPRX), which are known to play role in immune response. Finally, via structural modeling and molecular dynamics simulations, we hypothesized that the compact molecular architecture of StTGA2.1 distorts DNA conformation upon heterodimer binding to enable transcriptional activation. This study demonstrates how protein truncation can lead to distinct functions and that such events should be studied carefully in other protein families.

Ključne besede

proteini mini-TGA;rastlinski imunski odziv;transkripcijski faktorji TGA;virus krompirja Y;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 578
COBISS: 135092739 Povezava se bo odprla v novem oknu
ISSN: 0032-0889
Št. ogledov: 32
Št. prenosov: 7
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: proteini mini-TGA;rastlinski imunski odziv;transkripcijski faktorji TGA;virus krompirja Y;
Vrsta dela (COBISS): Članek v reviji
Komentar vira: Ostali avtorji: Marko Petek, Tjaša Lukan, Karmen Pogačar, Katja Stare, Erica Teixeira Prates, Daniel A Jacobson, Jan Zrimec, Gregor Bajc, Matej Butala, Maruša Pompe Novak, Quentin Dudley, Nicola Patron, Ajda Taler-Verčič, Aleksandra Usenik, Dušan Turk, Salomé Prat, Anna Coll, Kristina Gruden;
Strani: str. 1934–1952
Letnik: ǂVol. ǂ191
Zvezek: ǂiss. ǂ3
Čas izdaje: Mar. 2023
DOI: 10.1093/plphys/kiac579
ID: 24549651