Zala Kogej (Avtor), Marina Dermastia (Avtor), Nataša Mehle (Avtor)

Povzetek

Phytoplasmas of the 16SrIII group are wide spread, and have a broad plant host range. Among these, ‘Candidatus phytoplasma pruni’ (‘Ca. P. pruni’; phytoplasmas of 16SrIII subgroup A) can cause serious diseases in Prunus species and ‘Ca. P. pruni’-related strains can infect other plant species, including grapevines. In this study, a new real-time PCR detection system was developed for ‘Ca. P. pruni’ using TaqMan chemistry. This test was designed to detect ‘Ca. P. pruni’, by amplifying the species-specific secY gene. In addition, a test to amplify the group-specific 16S rRNA gene region was also developed. The performances of both tests were evaluated. The test that amplifies the secY gene provided reliable and quick detection of ‘Ca. P. pruni’. Using the newly developed and validated test, ‘Ca. P. pruni’ was not found in any of the 434 field samples collected from different plants species grown in different regions of Slovenia.

Ključne besede

rastline;fitoplazma;bolezni;PCR;phytoplasma;X-disease;real-time PCR;Prunus;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 632
COBISS: 24707587 Povezava se bo odprla v novem oknu
ISSN: 2076-0817
Št. ogledov: 24
Št. prenosov: 7
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarni naslov: Development and validation of a new TaqMan real-time PCR for detection of Candidatus phytoplasma pruni
Sekundarne ključne besede: rastline;fitoplazma;bolezni;PCR;
Komentar vira: Nasl. z nasl. zaslona; Opis vira z dne 10. 8. 2020;
Strani: str. 1-13
Letnik: ǂVol. ǂ9
Zvezek: ǂiss. ǂ8
Čas izdaje: 7 Aug. 2020
DOI: 10.3390/pathogens9080642
ID: 24574218