Andreja Kežar (Avtor), Luka Kavčič (Avtor), Martin Pólak (Avtor), Jiři Nováček (Avtor), Ion Gutierrez-Aguirre (Avtor), Magda Tušek-Žnidarič (Avtor), Anna Coll Rius (Avtor), Katja Stare (Avtor), Kristina Gruden (Avtor), Maja Ravnikar (Avtor), David Pahovnik (Avtor), Ema Žagar (Avtor), Franci Merzel (Avtor), Gregor Anderluh (Avtor), Marjetka Podobnik (Avtor)

Povzetek

Potato virus Y (PVY) is among the most economically important plant pathogens. Using cryoelectron microscopy, we determined the near-atomic structure of PVY’s flexuous virions, revealing a previously unknown lumenal interplay between extended carboxyl-terminal regions of the coat protein units and viral RNA. RNA–coat protein interactions are crucial for the helical configuration and stability of the virion, as revealed by the unique near-atomic structure of RNA-free virus-like particles. The structures offer the first evidence for plasticity of the coat protein’s amino- and carboxyl-terminal regions. Together with mutational analysis and in planta experiments, we show their crucial role in PVY infectivity and explain the ability of the coat protein to perform multiple biological tasks. Moreover, the high modularity of PVY virus-like particles suggests their potential as a new molecular scaffold for nanobiotechnological applications.

Ključne besede

plant pathogens;potato virus Y;viral RNA;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: KI - Kemijski inštitut
UDK: 577.2
COBISS: 5135183 Povezava se bo odprla v novem oknu
ISSN: 2375-2548
Št. ogledov: 32
Št. prenosov: 8
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Komentar vira: Nasl. z nasl. zaslona; Opis vira z dne 30. 7. 2019;
Strani: str. 1-13
Letnik: ǂVol. ǂ5
Zvezek: ǂno. ǂ7
Čas izdaje: 2019
DOI: 10.1126/sciadv.aaw3808
ID: 24579929