rapid molecular detection of virulence genes associated with avian pathogenic Escherichia coli in poultry
Polona Kogovšek (Avtor), Jerneja Ambrožič (Avtor), Alenka Dovč (Avtor), Tanja Dreo (Avtor), Hristo Hristov (Avtor), Uroš Krapež (Avtor), Maja Ravnikar (Avtor), Brigita Slavec (Avtor), Marjetka Lotrič (Avtor), Jana Žel (Avtor), Olga Zorman-Rojs (Avtor)

Povzetek

Infections with pathogenic Escherichia colican lead to different animal- and human-associated diseases. E. coliinfections are common in intensive poultry farming, and important economic losses can be expected during infections with avian pathogenic E. coli(APEC) strains followed by colibacillosis. Loop-mediated isothermal amplification (LAMP) assays were developed for rapid detection of 3 APEC-associated virulence genes: sitA, traT, and ompT. All 3 LAMP assays are shown to be specific, repeatable, and reproducible. High sensitivities of the assays are shown, where as few as 1,000 bacterial cells/mL can be detected in different matrices. On-site applicability of this LAMP method is demonstrated through testing of different sample types, from animal swabs and tissues, and from environmental samples collected from 6 commercial poultry farms. All 3 virulence genes were detected at high rates (above 85%) in samples from layer and broiler chickens with clinical signs and, interestingly, high prevalence of those genes was detected also in samples collected from clinically healthy broiler flock (above 75%) while lower prevalence was observed in remaining 3 clinically healthy chicken flocks (less than 75%). Importantly, these virulence genes were detected in almost all of the air samples from 11 randomly selected poultry houses, indicating air as an important route of E. colispread. Three LAMP assays that target APEC-associated virulence genes are shown to be sensitive and robust and are therefore applicable for rapid on-site testing of various sample types, from animal swabs to air. This on-site LAMP testing protocol offers rapid diagnostics, with results obtained in <35 min, and it can be applied to other important microorganisms to allow the required prompt measures to be taken.

Ključne besede

APEC virulence genes;isothermal amplification LAMP;on-site detection;colibacillosis;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL VF - Veterinarska fakulteta
UDK: 577.2
COBISS: 4878671 Povezava se bo odprla v novem oknu
ISSN: 0032-5791
Št. ogledov: 28
Št. prenosov: 6
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarne ključne besede: Escherichia coli;Poultry;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1500-1510
Letnik: ǂVol. ǂ98
Zvezek: ǂno. ǂ3
Čas izdaje: 2019
DOI: 10.3382/ps/pey516
ID: 24586393