structural characterization, antimicrobial activity, and DNA/BSA interactions

Povzetek

In this study, a tetradentate 1,3-propanediamine-N,N′-diacetate (1,3-pdda$^{2−}$) was utilized for the synthesis of a dinuclear gallium(III) complex, uns-cis-[Ga(1,3-pdda)(µ-OH)]$_2\cdot$2H$_2$O (1). Complex 1 was characterized using IR and NMR ($^1$H and $^{13}$C) spectroscopy, and its crystal structure was determined by single-crystal X-ray diffraction analysis. Both Ga(III) ions in Complex 1 exhibit octahedral geometry, with each ion coordinated by two nitrogen and two oxygen atoms from the 1,3-pdda$^{2–}$ ligand, as well as two oxygen atoms from the bridging hydroxyl groups. IR and NMR ($^1$H and $^{13}$C) spectra were simulated using DFT methods, showing a high degree of correlation with experimental data. Hirshfeld surface analysis provided insights into intermolecular interactions, with H⋯O and H⋯H interactions contributing significantly to the crystal stability. The antimicrobial potential of Complex 1 was evaluated alongside previously synthesized gallium(III) complexes, Na[Ga(1,3-pdta)]·3H$_2$O (2) and Ba[Ga(1,3-pndta)]2·3H$_2$O (3), with 1,3-pdta$^{4−}$ (1,3-propanediamine-N,N,N′,N′-tetraacetate) and 1,3-pndta$^{4−}$ ((±)-1,3-pentanediamine-N,N,N′,N′-tetraacetate), respectively. Among all the tested microbial species, the gallium(III) complexes have shown selective activity against Pseudomonas aeruginosa PAO1 strain and were able to reduce pyocyanin production by 40–43% in the clinical isolate BK25H of this bacterium. Moreover, Complexes 1–3 can modulate the quinolone-mediated quorum sensing system in P. aeruginosa PAO1. Interaction studies with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) were conducted to evaluate the binding affinity and mode of interaction of Complex 1 with key biomolecules, aiming to assess its potential for transport via serum proteins and its safety profile in terms of DNA interactions. Spectrofluorimetric experiments and molecular docking revealed that Complex 1 binds strongly to the Site I on BSA, with weaker interactions at the Site II. While spectrofluorimetric studies showed that Complex 1 has a slight affinity for minor groove binding or intercalation to ct-DNA, docking studies suggested some minor groove binding, especially in larger DNA sequences, with enhanced stabilization in 10-bp-DNA through hydrogen and carbon bonds.

Ključne besede

aminokarboksilatni ligand;protimikrobna aktivnost;interakcije DNA/BSA;galijevi(III) kompleksi;strukturna karakterizacija;aminocarboxylate ligand;antimicrobial activity;DNA/BSA interactions;gallium(III) complexes;structural characterization;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK: 546.681:547.466
COBISS: 233126403 Povezava se bo odprla v novem oknu
ISSN: 1565-3633
Št. ogledov: 93
Št. prenosov: 34
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: aminokarboksilatni ligand;protimikrobna aktivnost;interakcije DNA/BSA;galijevi(III) kompleksi;strukturna karakterizacija;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-25
Zvezek: ǂVol. ǂ2025, article ID 8097589
Čas izdaje: 14 Apr. 2025
DOI: 10.1155/bca/8097589
ID: 26230435
Priporočena dela:
, structural characterization, antimicrobial activity, and DNA/BSA interactions
, ǂthe ǂinfluence of an alkyl substituent in 1,3-propanediamine chain and the metal counter cation on the structural properties of the complex