an online tool for the analysis of circular dichroism spectroscopy data
Osvaldo Burastero (Avtor), Nykola C. Jones (Avtor), Lucas A. Defelipe (Avtor), Uroš Zavrtanik (Avtor), San Hadži (Avtor), Søren Vrønning Hoffmann (Avtor), Maria Garcia-Alai (Avtor)

Povzetek

Circular dichroism (CD) spectroscopy is an established biophysical technique to study chiral molecules. CD allows investigating conformational changes under varying experimental conditions and has been used to understand secondary structure, folding, and binding of proteins and nucleic acids. Here, we present ChiraKit, a user-friendly, online, and open-source tool to process raw CD data and perform advanced analysis. ChiraKit features include the calculation of protein secondary structure with the SELCON3 and SESCA algorithms, estimation of peptide helicity using the helix-ensemble model, the fitting of thermal/chemical unfolding or user-defined models, and the decomposition of spectra through singular value decomposition or principal component analysis. ChiraKit can be accessed at https://spc.embl-hamburg.de/.

Ključne besede

spektroskopija;cirkularni dikroizem;biofizikalna kemija;sekundarna struktura proteinov;termična denaturacija;intrinzično neurejeni proteini;programska oprema;spectroscopy;circular dichroism;biophysical chemistry;secondary structure;thermal unfolding;intrinsically disordered proteins;software;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK: 577.322:004.4
COBISS: 235365891 Povezava se bo odprla v novem oknu
ISSN: 1362-4962
Št. ogledov: 284
Št. prenosov: 71
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: spektroskopija;cirkularni dikroizem;biofizikalna kemija;sekundarna struktura proteinov;termična denaturacija;intrinzično neurejeni proteini;programska oprema;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-11
Letnik: ǂVol. ǂ
Zvezek: ǂiss. ǂ, [article no.] gkaf350
Čas izdaje: 2025
DOI: 10.1093/nar/gkaf350
ID: 26361774