Določevanje dresnikov (Fallopia sect. Reynoutria) z molekulskimi metodami

Povzetek

Ključne besede

Podatki

Jezik:	Slovenski jezik
Leto izida:	2012
Izvor:	Ljubljana
Tipologija:	2.11 - Diplomsko delo
Organizacija:	UL PEF - Pedagoška fakulteta
Založnik:	[M. Bjelić]
UDK:	582.657(043.2)
COBISS:	9388617
Št. ogledov:	903
Št. prenosov:	244
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Angleški jezik
Sekundarni naslov:	Determination of Fallopia sect. Reynoutria taxa with molecular methods
Sekundarni povzetek:	In our research invasive alien knotweed taxa sampled in Slovenia were identified using selected molecular methods. The aim was to find out whether Slovene samples of Fallopia sect. Reynoutria or sect. Sermentosae taxa can be unambiguously distinguished on the basis of the combination of chosen chloroplast and nuclear DNA markers. We used PCR RFLP analysis of the trnK intron of chloroplast DNA in combination with amplification of nuclear, simple sequence repeat (SSR) or microsatellite loci, KW6, potentially diagnostic F. sachalinensis-specific marker. According to the predominantly maternal inheritance of cpDNA in crosses of Fallopia species and based on PCR RFLP profiles of the trnK intron of cpDNA we could also make assumptions about the direction of hybridization between Japanese and giant knotweed. We tried to find out if the situation on this phenomenon in Slovenia was the same as in Europe, where hybridization between these two invasive alien knotweed species is unidirectional, with F. japonica (var. japonica), in European flora present as cytologically and genetically unified female clone, as the female (maternal) parent.
Sekundarne ključne besede:	botany;botanika;
Vrsta datoteke:	application/pdf
Vrsta dela (COBISS):	Diplomsko delo
Strani:	X, 70, [5] f.
Vrsta dela (ePrints):	thesis
Naslov (ePrints):	Determination of Fallopia sect. Reynoutria taxa with molecular methods
Ključne besede (ePrints):	invazivne tujerodne vrste
Ključne besede (ePrints, sekundarni jezik):	invasive alien species
Povzetek (ePrints):	V okviru diplomskega dela smo z izbranimi molekulskimi metodami določali taksone invazivnih tujerodnih dresnikov, nabranih v Sloveniji. Želeli smo ugotoviti, ali lahko na podlagi kombinacije izbranih molekulskih markerjev kloroplastne (cp) in jedrne (n) DNA razlikujemo med slovenskimi vzorci rastlin iz rodu Fallopia sect. Reynoutria oz. sect. Sermentosae. Na vzorcih rastlin obravnavanega rodu smo izvedli PCR-RFLP-analizo introna trnK kloroplastne DNA in z reakcijo PCR pomnoževali potencialno diagnostični in za sahalinski dresnik specifični marker jedrne DNA, mikrosatelitski lokus KW6. Upoštevajoč maternalno dedovanje cpDNA pri predstavnikih rodu Fallopia lahko na podlagi PCR-RFLP-profilov introna trnK cpDNA analiziranih vzorcev invazivnih tujerodnih dresnikov ugotovimo tudi smer hibridizacije med japonskim in sahalinskim dresnikom. Naša raziskava je pokazala, da je tudi pri nas, tako kot širše v Evropi, križanje med tema dvema vrstama enosmerno in da je japonski dresnik, ki je v evropski flori prisoten kot citološko in genetsko enoten ženski klon, v primeru križanja vedno v vlogi materinske (ženske) rastline.
Povzetek (ePrints, sekundarni jezik):	In our research invasive alien knotweed taxa sampled in Slovenia were identified using selected molecular methods. The aim was to find out whether Slovene samples of Fallopia sect. Reynoutria or sect. Sermentosae taxa can be unambiguously distinguished on the basis of the combination of chosen chloroplast and nuclear DNA markers. We used PCR RFLP analysis of the trnK intron of chloroplast DNA in combination with amplification of nuclear, simple sequence repeat (SSR) or microsatellite loci, KW6, potentially diagnostic F. sachalinensis-specific marker. According to the predominantly maternal inheritance of cpDNA in crosses of Fallopia species and based on PCR RFLP profiles of the trnK intron of cpDNA we could also make assumptions about the direction of hybridization between Japanese and giant knotweed. We tried to find out if the situation on this phenomenon in Slovenia was the same as in Europe, where hybridization between these two invasive alien knotweed species is unidirectional, with F. japonica (var. japonica), in European flora present as cytologically and genetically unified female clone, as the female (maternal) parent.
Ključne besede (ePrints, sekundarni jezik):	invasive alien species
ID:	8310794