Priprava plazmidnih konstruktov za preučitev promotorske aktivnosti genov bakterije Aggregatibacter actinomycetemcomitans

Povzetek

Ključne besede

Podatki

Jezik:	Slovenski jezik
Leto izida:	2013
Izvor:	Ljubljana
Tipologija:	2.11 - Diplomsko delo
Organizacija:	UL PEF - Pedagoška fakulteta
Založnik:	[I. Šurla]
UDK:	616.31:579.8(043.2)
COBISS:	9786185
Št. ogledov:	874
Št. prenosov:	219
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Angleški jezik
Sekundarni naslov:	Construction of plasmids to study promotor activity of genes of bacteria Aggregatibacter actinomycetemcomitans
Sekundarni povzetek:	Aggregatibacter actinomycetemcomitans is a bacterium that causes aggressive and chronic parodontitis. The main effect of this periodontal disease is a collapse of periodontal tissues in a very short period of time. This is caused by virulent factors that the bacteria excrete in periodontal pockets. Most known factors are leukotoxin and toxin CDT (Cytolethal distending toxin). In my thesis I have focused on the bacteria A. actinomycetecomitans and associated it with parodontitis disease. Most of my work consisted of the preparation of plasmid construct to examine the promoter activity for the biosynthesis of leukotoxin and toxin CDT in bacteria. By engineering the recombinant plasmid that harbours the promoter region of the toxin CDT or of the leukotoxin fused to the β-galactosidase reporter gene we can recognize the transcriptional factors that are important for regulation of the gene expression and therefore determine the environmental signals needed for the induction of either toxin.
Sekundarne ključne besede:	biochemistry;biokemija;
Vrsta datoteke:	application/pdf
Vrsta dela (COBISS):	Diplomsko delo/naloga
Komentar na gradivo:	Univ. Ljubljana, Pedagoška fak., Biologija in kemija
Strani:	III, 32 str.
Vrsta dela (ePrints):	thesis
Naslov (ePrints):	Construction of plasmids to study promotor activity of genes of bacteria Aggregatibacter actinomycetemcomitans
Ključne besede (ePrints):	Aggregatibacter actinomycetemcomitans
Ključne besede (ePrints, sekundarni jezik):	Aggregatibacter actinomycetemcomitans
Povzetek (ePrints):	Aggregatibacter actinomycetemcomitans je bakterija, ki povzroča agresivni in kronični parodontitis. Glavna posledica te parodontalne bolezni je propad obzobnih tkiv v zelo kratkem času. Bolezensko stanje se pojavi zaradi virulenčnih faktorjev, ki jih bakterija izloča v parodontalne žepe, med katerimi sta najbolj znana toksina CDT (ang. Cytolethal distending toxin) in levkotoksin. V diplomskem delu sem se posvetila bakteriji A. actimomycetemcomitans ter jo povezala z boleznijo parodontitis. Osredotočila sem se na pripravo plazmidnih konstruktov za preučitev promotorske aktivnosti za biosintezo levkotoksina in toksina CDT v bakteriji. S plazmidnim konstruktom, ki vsebuje promotorsko regijo toksina CDT ali levkotoksina spojenega s poročevalskim genom za β-galaktozidazo, lahko določimo dejavnike, ki vplivajo na izražanje genov teh toksinov. Posledično lahko določimo signale okolja, ki so pomembni za sintezo teh toksinov.
Povzetek (ePrints, sekundarni jezik):	Aggregatibacter actinomycetemcomitans is a bacterium that causes aggressive and chronic parodontitis. The main effect of this periodontal disease is a collapse of periodontal tissues in a very short period of time. This is caused by virulent factors that the bacteria excrete in periodontal pockets. Most known factors are leukotoxin and toxin CDT (Cytolethal distending toxin). In my thesis I have focused on the bacteria A. actinomycetecomitans and associated it with parodontitis disease. Most of my work consisted of the preparation of plasmid construct to examine the promoter activity for the biosynthesis of leukotoxin and toxin CDT in bacteria. By engineering the recombinant plasmid that harbours the promoter region of the toxin CDT or of the leukotoxin fused to the β-galactosidase reporter gene we can recognize the transcriptional factors that are important for regulation of the gene expression and therefore determine the environmental signals needed for the induction of either toxin.
Ključne besede (ePrints, sekundarni jezik):	Aggregatibacter actinomycetemcomitans
ID:	8311718