Disodium guanosine 5'-monophosphate self-associates into nanoscale cylinders at pH 8: a combined diffusion NMR spectroscopy and dynamic light scattering study

Alan Wong (Avtor), Ida Ramsey (Avtor), Lea Spindler (Avtor), Gang Wu (Avtor)

Povzetek

We report a combined NMR and dynamic light scattering (DLS) study on the size of supramolecular structures formed by disodium guanosine 5'-monophosphate, Na2(5'-GMP), at pH 8. In general, two distinct types of aggregate species are present in an aqueous solution of Na2(5'-GMP). One type consists of stacking 5'-GMP monomers, and the other contains stacking G-quartets. Both types of aggregates can be modeled as rodlike cylinders. The cylinder diameter is 10 and 26 A for monomer aggregates and quartet aggregates, respectively. For Na2(5'-GMP) concentrations between 18 and 34 wt %, the cylinders formed by stacking G-quartets have an average length between 8 and 30 nm, corresponding to a stack of ~24-87 G-quartets. These nanoscale aggregates are significantly larger than what had previously been believed for Na2(5'-GMP) self-associationat pH 8. The length of both types of 5'-GMP aggregates was found to increase with Na2(5ć-GMP) concentration but was insensitive to the added NaCl in solution. While the aggregate size for monomer aggregates increases with a decrease in temperature, the size of G-quartet aggregates is essentially independent of temperature. We found that the size of G-quartet aggregates is slightly larger in D2O than in H2O, whereas the size of monomer aggregates remains the same in D2O and in H2O. We observed a linear relationship between the axial ratio of the 5ć-GMP cylinders and the Na2(5'-GMP) concentration for both types of 5ć-GMP aggregates, which suggests a common stacking mechanism for monomers and G-quartets.

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Podatki

Jezik:	Angleški jezik
Leto izida:	2005
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UM FS - Fakulteta za strojništvo
UDK:	539:544
COBISS:	9783574
ISSN:	0002-7863
Št. ogledov:	993
Št. prenosov:	78
Ocena:	0 (0 glasov)
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Ostali podatki

Sekundarni jezik:	Angleški jezik
URN:	URN:SI:UM:
Strani:	str. 6990-6998
Letnik:	ǂVol. ǂ127
Zvezek:	ǂno. ǂ19
Čas izdaje:	2005
ID:	8718315