Sergej Tomić (Avtor), Rebeka Rudolf (Avtor), Mihael Brunčko (Avtor), Ivan Anžel (Avtor), V. Savić (Avtor), Miodrag Čolić (Avtor)

Povzetek

Ni-Ti Shape Memory Alloys (SMAs) have attracted considerable attention as biomaterials for medical devices. However, the biocompatibility of Ni-Ti SMAs is often unsatisfactory due to their poor surface structure. Here we prepared Rapidly Solidifi ed (RS) Ni-Ti SMA ribbons by melt-spinning and their surface was characterised by Augerelectron spectroscopy, X-ray photoelectron spectrometry and scanning electron microscopy. The biocompatibility of the produced ribbons and their immunomodulatory properties were studied on human monocyte-derived dendritic cells (MoDCs). We showed that melt-spinning of Ni-Ti SMAs can form a thin homogenous oxide layer, which improves their corrosion resistance and subsequent toxicity to MoDCs. Ni-Ti RS ribbons stimulated the maturation of MoDCs, as detected by changes in the cellsć morphology and increased expression of HLA-DR, CD86, CD40 and CD83 molecules. However, Ni-Ti RS ribbons enhanced the tolerogenic properties of immature MoDCs, which produced higher levels of IL-10 and IL-27, driving the differentiation of IL-10- and TGF-â-producing CD4+T cells. On the other hand, in the presence of lipopolysaccharide, an important pro-infl ammatory biomolecule, Ni-Ti RS ribbons enhanced the allostimulatory and Th1 polarising capacity of MoDCs, whereas the production of Th2 and Th17 cytokines was down-regulated. In conclusion, Ni- Ti RS ribbons possess substantial immunomodulatory properties on MoDCs. These fi ndings might be clinically relevant, because implanted Ni-Ti SMA devices can induce both desired and adverse effects on the immune system, depending on the microenvironmental stimuli.

Ključne besede

nickel-titanium alloy;biocompatibility;cytokines;immunomodulation;monocyte-derived dendritic cells;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM FS - Fakulteta za strojništvo
UDK: 669:61
COBISS: 15705366 Povezava se bo odprla v novem oknu
ISSN: 1473-2262
Št. ogledov: 1651
Št. prenosov: 328
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: nikelj-titanova zlitina;biokompatibilnost;citokini;imunomodulacija;monocitne dendritične celice;
URN: URN:SI:UM:
Vrsta dela (COBISS): Znanstveno delo
Strani: str. 58-80
Zvezek: ǂVol. ǂ23
Čas izdaje: Jan.-June 2012
DOI: 10.22203/eCM.v023a05
ID: 8718484