Jana Ambrožič-Dolinšek (Avtor), Marjana Camloh (Avtor), Jana Žel (Avtor), Maja Kovač (Avtor), Maja Ravnikar (Avtor), Luigi Carraro (Avtor), Nataša Petrovič (Avtor)

Povzetek

The aim of the work was to infect pyrethrum (Tanacetum cinerariifolium (Trevir.) Schulty-Bip.), an important secondary metabolite pyrethrin producing plant, with MA phytoplasma (16S rRNA group III, subgroup B) to initiate tissue culture of phytoplasma-infected plants, and to examine possible effects of infection on pyrethrin content, morphology, and regenerative capacity in shoot culture. Phytoplasma transmission was achieved bz using an insect vector (Euscelidius variegatus) making pyrethrum as a new host for phytoplasma. The infection significantly changed the morphology and regenerative capacity of shoots and roots. The infected shoots and roots were shorter and thinner and an increase in proliferation of both was observed. Phytoplasma infection significantly decreased pyrethrin content in shoots, as measured by HPLC. The possibility of long-term maintenance of phytoplasma was also studied. The presence of phytoplasma in shoots was confirmed periodically over 4 years by PCR assay. Possible physiological reasons for a balanced phytoplasma-host system are discussed.

Ključne besede

Tanacetum cinerariifolium;pyrethrum;phytoplasma infection;shoot culture;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 577
COBISS: 1842511 Povezava se bo odprla v novem oknu
ISSN: 0304-4238
Št. ogledov: 1488
Št. prenosov: 107
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
URN: URN:SI:UM:
Vrsta dela (COBISS): Delo ni kategorizirano
Strani: str. 213-218
Letnik: ǂVol. ǂ116
Zvezek: ǂno. ǂ2
Čas izdaje: 2008
ID: 8724005