Sekundarni povzetek: |
The topic of following dissertation is the development and validity of high-performance liquid chromatography (HPLC-UV/VIS), which is the method of analysis for determining non-steroid anti-inflammatory substances. Furthermore, it discusses the transfer of developed method on LC-ESI-MSMS, where different procedures of sample preparation are the ones, which influence our choice of analysis system for qualification and quantification. A lot of attention was given to different procedures of preparing such dressings, which are connected to analgesics (painkillers) that belong to the group of non-steroidal anti-rheumatics (NSAID).
The characteristic of the alginate dressings is that after applying them on the spot of skin inflammation, they are formed on the wound or inflammation, thereby optimal skin moisture is achieved, and physical-chemical characteristics cause faster healing and hemostatic effect. By putting non-steroid anti-inflammatory substances in high-absorbing non-woven fabric dressings, we wished to achieve slow and controlled release on the spot of inflammation. The results show that we do not achieve the wanted release by implementing the procedures of applying naproxen on the alginate dressing. However, with the procedures of modification of the primary method the even release can be achieved. Moreover, for the quantification of the released naproxen from dressings prepared that way by implementing the method 2, with the procedures of validity of the method, we managed to check, whether the method gives correct and accurate results, and is therefore suitable for routine prescription of non-steroid anti-inflammatory substances. The mobile phase and other parameters at development of the analysis of the method 2 were selected two-dimensionally, since the method can be useful on HPLC-UV/VIS as well as on LC-ESI-MSMS. The choice of system which is going to be used, depends mainly of the concentration area of the released naproxen from the alginate dressings. However, this depends mainly of the manner of preparation. In case of release from the alginate dressings, the system LC-ESI-MSMS is suitable mainly in the manner MRM, where even the precisely determined fragment, m/z 231, can be qualified and quantified in a sample. |