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Gene expression profiling of recombinant protein producing E. coli at suboptimal growth temperature
Mitja Mahnič, Špela Baebler, Andrej Blejec, Špela Jalen, Kristina Gruden, Viktor Menart, Simona Jevševar
Izvirni znanstveni članek
Oznake: recombinant protein production;non-classical inclusion bodies;expression microarrays;YaeL protease;GroEL chaperone;
Recent studies have revealed that at lower cultivation temperatures (25 °C) much higher percentage of correctly folded recombinant hG-CSF protein can be extracted from inclusion bodies. Hence, the goal of our research was to investigate mechanisms determining characteristics of non-classical inclusi ...
Leto: 2012 Vir: Nacionalni inštitut za biologijo (NIB)
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