Št. zadetkov: 13
Video in druga učna gradiva
Oznake:
science;scientific research;biology
Leto:
2017
Vir:
videolectures.net
Magistrsko delo
Oznake:
dPCR;kontrolni material;virus opičjih koz;
Za zanesljivo diagnostiko z molekularnimi metodami potrebujemo kontrolne materiale, ki med drugim omogočajo primerjavo rezultatov med laboratoriji. Uporaba kontrolnih materialov je zelo pomembna pri razvoju novih testov, natančni kvantifikaciji genetskega materiala, z njimi tudi upoštevamo in kompen ...
Leto:
2024
Vir:
Fakulteta za kemijo in kemijsko tehnologijo (UL FKKT)
Izvirni znanstveni članek
Oznake:
nukleinske kisline;dPCR;metode ekstrakcije DNK;gram-negativne bakterije;nucleic acid;DNA extraction methods;Gram-negative bacteria;
Detection and quantification of DNA biomarkers relies heavily on the yield and quality of DNA obtained by extraction from different matrices. Although a large number of studies have compared the yields of different extraction methods, the repeatability and intermediate precision of these methods hav ...
Leto:
2023
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
digital PCR;pepino mosaic virus;quantification;genotype specific;
In recent years, pepino mosaic virus (PepMV) has rapidly evolved from an emerging virus to an endemic pathogen, as it causes significant loses to tomato crops worldwide. At present, the main control strategy for prevention of PepMV disease in tomato production remains based on strict hygiene measure ...
Leto:
2020
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
genetically modified organisms;digital PCR;GMO quantification;complex matrices;
The increased use of genetically modified organisms (GMOs) is accompanied by increased complexity of the matrices that contain GMOs. The most common DNA-based approach for GMO detection and quantification is real-time quantitative polymerase chain reaction (qPCR). However, as qPCR is sensitive to in ...
Leto:
2019
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
droplet digital PCR;genetically modified organisms;soybean;
Quantification of genetically modified organisms (GMOs) in food and feed products is often required for their labelling or for tolerance thresholds. Standard-curve-based simplex quantitative polymerase chain reaction (qPCR) is the prevailing technology, which is often combined with screening analysi ...
Leto:
2017
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
digitalna PCR;Gram-negativne bakterije;detekcija patogenov;okužba dihal;biomarkerji;diagnostika;molekularna biologija;digital PCR (dPCR);Gram-negative bacteria;pathogen detection;respiratory infections;biomarkers;RUCS algorithm;
Rapid and accurate identification of bacterial pathogens is crucial for effective treatment and infection control, particularly in hospital settings. Conventional methods like culture techniques and MALDI-TOF mass spectrometry are often time-consuming and less sensitive. This study addresses the nee ...
Leto:
2024
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
digitalna PCR;digitalna verižna reakcija s polimerazo;kvantifikacija;multipleksiranje;gensko spremenjeni organizmi;6-barvni sistem;diagnostika virusov;virologija;digital PCR;dPCR;quantification;multiplexing;genetically modified organisms;6-color system;virus diagnostics;virology;
The proliferation of genetically modified organisms (GMOs) presents challenges to GMO testing laboratories and policymakers. Traditional methods, like quantitative real-time PCR (qPCR), face limitations in quantifying the increasing number of GMOs in a single sample. Digital PCR (dPCR), specifically ...
Leto:
2023
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
protimikrobna rezistenca;HCMV;verižna reakcija s polimerazo;virusi;digital PCR;antimicrobial-drug resistance;polymerase chain reaction;viruses;
Antimicrobial drug resistance is one of the biggest threats to human health worldwide. Timely detection and quantification of infectious agents and their susceptibility to antimicrobial drugs are crucial for efficient management of resistance to antiviral drugs. In clinical settings, viral drug resi ...
Leto:
2020
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
tarčno visokozmogljivo sekvenciranje;bakterijski mikrobiom;rezistom;detekcija bakterij;izolacija DNA;meroslovje;diagnostika;ponovljivost;targeted high-throughput sequencing;bacterial microbiome;resistome;bacteria detection;DNA extraction;metrology;diagnostics;repeatability;
Targeted high-throughput sequencing (HTS) has revolutionized the way we look at bacterial communities. It can be used for the species-specific detection of bacteria as well as for the determination of the microbiome and resistome and can be applied to samples from almost any environment. However, th ...
Leto:
2024
Vir:
Nacionalni inštitut za biologijo (NIB)