Št. zadetkov: 17
Izvirni znanstveni članek
Oznake:
teeth;analysisi of DNA preservation;forensic research;archeological research;zobje;analiza ohranjenosti DNA;forenzična raziskava;arheološka raziskava;
This study investigates the preservation of DNA in different categories of teeth, including permanent and deciduous, fully developed and not fully developed, in both adults and non-adults. Teeth were sampled from a modern-era cemetery in Ljubljana, Slovenia. DNA extraction was performed using a full ...
Leto:
2023
Vir:
Filozofska fakulteta (UL FF)
Izvirni znanstveni članek
Oznake:
aged DNA;compact bone;DNA preservation;human identification;trabecular bone;
Significant variation exists in the molecular structure of compact and trabecular bone. In compact bone full dissolution of the bone powder is required to efficiently release the DNA from hydroxyapatite. In trabecular bone where soft tissues are preserved, we assume that full dissolution of the bone ...
Leto:
2024
Vir:
Medicinska fakulteta (UL MF)
Izvirni znanstveni članek
Oznake:
skeletal remains;ancient DNA;petrous bones;
Phenotypic trait prediction in ancient DNA analysis can provide information about the external appearance of individuals from past human populations. Some studies predicting eye and hair color in ancient adult skeletons have been published, but not for ancient subadult skeletons, which are more pron ...
Leto:
2023
Vir:
Medicinska fakulteta (UL MF)
Izvirni znanstveni članek
Oznake:
Second World War;skeletal remains;mtDNA;nDNA;qPCR;
Mitochondrial DNA (mtDNA) is of great value in forensics to procure information about a person when a next of kin, personal belongings, or other sources of nuclear DNA (nDNA) are unavailable, or nDNA is lacking in quality and quantity. The quality and reliability of the results depend greatly on ens ...
Leto:
2023
Vir:
Medicinska fakulteta (UL MF)
Kratki znanstveni prispevek
Oznake:
haplotype;library input;mitogenome;next-generation sequencing;Second World War;
Optimizing analysis parameters and sample input is crucial in forensic genetics methods to generate reliable results, and even more so when working with muti-copy mitochondrial DNA (mtDNA) and low-quality samples. This study compared mitotypes based on next-generation sequencing (NGS) results derive ...
Leto:
2023
Vir:
Medicinska fakulteta (UL MF)
Izvirni znanstveni članek
Oznake:
ATR-FTIR spectroscopy;skeletal remains;bone maturation;collagen;mineral;DNA preservation;
Molecular characteristics of skeletal remains were studied utilizing ATR-FTIR spectroscopy, focusing on comparisons between mature adult and immature non-adult skeletal elements. To cover the intra-skeletal variability, different types of bones were analysed. The objective was to identify significan ...
Leto:
2024
Vir:
Filozofska fakulteta (UL FF)
Izvirni znanstveni članek
Oznake:
ATR-FTIR spectroscopy;DNA preservation;metacarpals;molecular structure;petrous bone;soft tissue remnants;
A comparative analysis of 26 petrous bones and epiphyses of metacarpals from the Second World War era revealed no significant differences in DNA yield or success in STR typing. This unexpected parity in DNA preservation between the petrous bone, a renowned source of endogenous DNA in skeletal remain ...
Leto:
2024
Vir:
Filozofska fakulteta (UL FF)
Izvirni znanstveni članek
Oznake:
ekstrakcija kostne DNK;starodavna DNK;identifikacija pogrešane osebe;bone DNA extraction;ancient DNA;missing person identification;
An efficient extraction method is important for obtaining high-quality DNA from degraded aged bone samples. An automated full-demineralization method using the EDTA and DNA Investigator Kit (Qiagen) combined with Qiagen’s biorobots was optimized in our laboratory in the past to extract the DNA from ...
Leto:
2023
Vir:
Medicinska fakulteta (UL MF)
Izvirni znanstveni članek
Oznake:
bone DNA;SNP analysis;DNA degradation;
PCR-MPS is an emerging tool for the analysis of low-quality DNA samples. In this study, we used PCR-MPS to analyse 32 challenging bone DNA samples from three Second World War victims, which previously yielded no results in conventional STR PCR-CE typing. The Identity Panel was used with 27 cycles of ...
Leto:
2023
Vir:
Medicinska fakulteta (UL MF)
Izvirni znanstveni članek
Oznake:
analiza sorodstva;starodavna DNK;petrol kosti;kinship analysis;ancient DNA;petrous bones;
The familial relationship between skeletons buried together in a shared grave is important for understanding the burial practices of past human populations. Four skeletons were excavated from the Late Antiquity part of the Bled–Pristava burial site in Slovenia, dated to the 5th to 6th century. They ...
Leto:
2023
Vir:
Medicinska fakulteta (UL MF)