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Small RNA sequencing and assembly: reconstructing viral genomes from defence molecules
Jan Kreuze
Video in druga učna gradiva
Oznake: biology;molecular biology
RNA silencing constitutes a fundamental antiviral defence mechanism in plants in which host enzymes cut viral RNA into pieces of 20-24 nucleotides. When isolated, sequenced en mass and properly assembled or aligned these virus-derived small RNA (sRNA) sequences can reconstitute genomic sequence info ...
Leto: 2013 Vir: videolectures.net
Salicylic acid perturbs sRNA-gibberellin regulatory network in immune response of potato to Potato virus Y infection
Maja Križnik, Marko Petek, David Dobnik, Živa Ramšak, Špela Baebler, Stephan Pollmann, Jan F. Kreuze, Jana Žel, Kristina Gruden
Izvirni znanstveni članek
Oznake: gibberellin;miRNA/siRNA;plant immunity;potato;Potato virus Y;salicylic acid;symbiosis;tolerance;
Potato virus Y is the most economically important potato viral pathogen. We aimed at unraveling the roles of small RNAs (sRNAs) in the complex immune signaling network controlling the establishment of tolerant response of potato cv. Désirée to the virus. We constructed a sRNA regulatory network conn ...
Leto: 2017 Vir: Nacionalni inštitut za biologijo (NIB)
Interlaboratory comparison study on ribodepleted total RNA high-throughput sequencing for plant virus diagnostics and bioinformatic competence
Yahya Gaafar, Marcel Westenberg, Marleen Botermans, László Krizbai, Kris De Jonghe, Yoika Foucart, Luca Ferretti, Denis Kutnjak, Anja Pecman, Nataša Mehle, Jan F. Kreuze, Giovanna Müller, Nikolaos Vakirlis, Despoina Beris, Christina Varveri, Heiko Ziebell
Izvirni znanstveni članek
Oznake: high-throughput sequencing;ribodepletion;interlaboratory comparison;test performance study;proficiency test;Virtool;
High-throughput sequencing (HTS) technologies and bioinformatic analyses are of growing interest to be used as a routine diagnostic tool in the field of plant viruses. The reliability of HTS workflows from sample preparation to data analysis and results interpretation for plant virus detection and i ...
Leto: 2021 Vir: Nacionalni inštitut za biologijo (NIB)
ǂA ǂprimer on the analysis of high-throughput sequencing data for detection of plant viruses
Denis Kutnjak, Lucie Tamisier, Ian Adams, Neil Boonham, Thierry Candresse, Michela Chiumenti, Kris De Jonghe, Jan F. Kreuze, Marie Lefebvre, Goncalo Silva, Martha Malapi-Wight, Paolo Margaria, Irena Mavrič Pleško, Sam McGreig, Laura Miozzi, Benoit Remenant, Jean-Sébastien Reynard, Johan Rollin, Mike Rott, Olivier Schumpp, Sébastien Massart, Annelies Haegeman
Pregledni znanstveni članek
Oznake: varstvo rastlin;rastlinski virus;bioinformatika;mikroorganizmi;zaznavanje;odkritje;plant virus;high-throughput sequencing;bioinformatics;detection;discovery;
High-throughput sequencing (HTS) technologies have become indispensable tools assisting plant virus diagnostics and research thanks to their ability to detect any plant virus in a sample without prior knowledge. As HTS technologies are heavily relying on bioinformatics analysis of the huge amount of ...
Leto: 2021 Vir: Nacionalni inštitut za biologijo (NIB)
Št. zadetkov: 4
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