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Št. zadetkov: 5
Digital PCR for the characterization of reference materials
Megan H. Cleveland, Hua-Jun He, Mojca Milavec, Young-Kyung Bae, Peter M. Vallone, Jim F. Huggett
Pregledni znanstveni članek
Oznake: digitalna PCR;digitalna verižna reakcija s polimerazo;referenčni materiali;UV-spektroskopija;digital PCR;dPCR;reference materials;UV-spectroscopy;
Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characteri ...
Leto: 2024 Vir: Nacionalni inštitut za biologijo (NIB)
Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA
Jernej Pavšič, Alison S. Devonshire, Andrej Blejec, Carole A. Foy, Fran Van Heuverswyn, Gerwyn M. Jones, Heinz Schimmel, Jana Žel, Jim F. Huggett, Nicholas Redshaw, Maria Karczmarczyk, Erkan Mozioglu, Sema Akyürek, Müslüm Akgöz, Mojca Milavec
Izvirni znanstveni članek
Oznake: digital PCR;DNA quantification;inter-laboratory assessment;human cytomegalovirus;virus reference materials;
Quantitative PCR (qPCR) is an important tool in pathogen detection. However, the use of different qPCR components, calibration materials and DNA extraction methods reduces comparability between laboratories, which can result in false diagnosis and discrepancies in patient care. The wider establishme ...
Leto: 2017 Vir: Nacionalni inštitut za biologijo (NIB)
Survey results on nucleic acid tests of infectious diseases
Jörg Neukammer, Martin Hussels, Andreas Kummrow, Alison S. Devonshire, Carole A. Foy, Jim F. Huggett, Helen C. Parkes, Jana Žel, Mojca Milavec, Heinz Schimmel, Wolfgang Unger, Müslüm Akgöz, Timothy D. McHugh, Viktorija Tomič, Hans-Peter Grunert, Heinz Zeichhardt
Izvirni znanstveni članek
Oznake: nucleic acid tests;infectious diseases;virus detection;bacteria detection;isothermal nucleic acid amplification;status report;questionnaire;NAT;PCR;
This survey discusses current and emerging isothermal and rapid polymerase chain reaction (PCR) based nucleic acid amplification methods for near-patient diagnostics. To assess the clinical need of rapid diagnostics for infectious diseases based on nucleic acid tests (NATs) we performed and analy ...
Leto: 2015 Vir: Nacionalni inštitut za biologijo (NIB)
ǂThe ǂperformance of human cytomegalovirus digital PCR reference measurement procedure in seven external quality assessment schemes over four years
Mojca Milavec, Jernej Pavšič, Alexandra Bogožalec Košir, Gerwyn M. Jones, Denise M. O'Sullivan, Alison S. Devonshire, Fran Van Heuverswyn, Maria Karczmarczyk, Jannika Neeb, Annabell Plauth, Philippe Corbisier, Heinz Schimmel, Andreas Kummrow, Jörg Neukammer, Carole A. Foy, Martin Kammel, Hans-Peter Grunert, Heinz Zeichhardt, Jim F. Huggett
Izvirni znanstveni članek
Oznake: biokemija;digitalni PCR;referenčne meritve;virusi;
Leto: 2022 Vir: Nacionalni inštitut za biologijo (NIB)
ǂThe ǂdigital MIQE guidelines update
Jim F. Huggett, Alexandra S. Whale, Ward De Spiegelaere, Afif M. Abdel Nour, Young-Kyung Bae, Vladimír Beneš, Dan Burke, Megan Cleveland, Philippe Corbisier, Alison S. Devonshire, Lianhua Dong, Daniela Drandi, Carole A. Foy, Jeremy A. Garson, Hua-Jun He, Jan Hellemans, Mikael Kubista, Antoon Lievens, Mike G. Makrigiorgos, Mojca Milavec, Reinhold D. Mueller, Tania Nolan, Denise M. O'Sullivan, Michael W. Pfaffl, Stefan Rödiger, Erica L. Romsos, Gregory L. Shipley, Valérie Taly, Andreas Untergasser, Carl T. Wittwer, Stephen A. Bustin, Jo Vandesompele
Pregledni znanstveni članek
Oznake: biokemija;digitalna PCR;dPCR;MIQE;dMIQE;
Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments ...
Leto: 2020 Vir: Nacionalni inštitut za biologijo (NIB)
Št. zadetkov: 5
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