Št. zadetkov: 13
Pregledni znanstveni članek
Oznake:
kombinatorna knjižnica;peptidna knjižnica;peptide;combinatorial library;library design;screening;mutagenesis;
Peptides are widely used in pharmaceutical industry as active pharmaceutical ingredients, versatile tools in drug discovery, and for drug delivery. They find themselves at the crossroads of small molecules and proteins, possessing favorable tissue penetration and the capability to engage into specif ...
Leto:
2020
Vir:
Fakulteta za farmacijo (UL FFA)
Pregledni znanstveni članek
Oznake:
constrained peptides;macrocycles;crosslinking;enzymatic cyclization;stapling;hairpin loop;
The sheer size and vast chemical space (i.e., diverse repertoire and spatial distribution of functional groups) underlie peptides' ability to engage in specific interactions with targets of various structures. However, the inherent flexibility of the peptide chain negatively affects binding affinity ...
Leto:
2021
Vir:
Fakulteta za farmacijo (UL FFA)
Doktorska disertacija
Oznake:
peptidni ligandi;monoklonska protitelesa;predstavitev na bakteriofagu;usmerjena knjižnica;afinitetna kromatografija;imunoglobulini IgG;
Monoklonska protitelesa (mAb) izotipa IgG uporabljamo kot biološke učinkovine za zdravljenje rakavih obolenj, , avtoimunih in kroničnih vnetnih, infekcijskih in številnih drugih bolezni. mAb široko uporabljamo tudi v diagnostiki v različnih formatih predvsem zaradi njihove izjemne specifičnosti in v ...
Leto:
2021
Vir:
Fakulteta za farmacijo (UL FFA)
Magistrsko delo
Oznake:
Ciklični peptidi;ligandi;afiniteta vezave;predstavitev na bakteriofagu;ELISA;
Peptidi so skupina molekul, sestavljenih iz dveh ali več aminokislinskih ostankov, povezanih s peptidno vezjo. So vsestransko uporabni, med drugim igrajo pomembno vlogo afinitetnih ligandov imunoglobulinov (Ig) in so dobra alternativa naravnim Ig-vezavnim proteinom. Ker imajo bolj preprosto zgradbo ...
Leto:
2022
Vir:
Fakulteta za farmacijo (UL FFA)
Diplomsko delo
Oznake:
afimeri;Clostridioides difficile;čiščenje;DARPini;endolizini;izražanje;vezalci eksotoksinov;
Zdravljenje okužbe z bakterijo Clostridioides difficile zaradi odpornosti proti antibiotikom predstavlja veliko problematiko v sodobnem svetu. Do okužb prihaja zlasti v bolnišničnem okolju ob uporabi antibiotikov, ki izzovejo spremembe v sestavi črevesne mikrobiote. C. difficile povzroča vnetja in k ...
Leto:
2023
Vir:
Fakulteta za farmacijo (UL FFA)
Pregledni znanstveni članek
Oznake:
Chinese hamster ovary cells;cell line development;CRISPR-Cas;genome editing;glycoengineering;augmenting productivity;
The Chinese hamster ovary (CHO) cell line is a well-established platform for the production of biopharmaceuticals due to its ability to express complex therapeutic proteins with human-like glycopatterns in high amounts. The advent of CRISPR technology has opened up new avenues for the engineering of ...
Leto:
2023
Vir:
Fakulteta za farmacijo (UL FFA)
Izvirni znanstveni članek
Oznake:
antibacterial agents;DNA-encoded library;screening;inhibitors;
Screening of DNA-encoded libraries is an emerging technology for discovering hits against protein targets. With the recent launch of the DELopen platform, a facile screening of 4.4 billion compounds is available to accelerate the drug discovery process. Here we report an affinity-based screening of ...
Leto:
2022
Vir:
Fakulteta za farmacijo (UL FFA)
Izvirni znanstveni članek
Oznake:
peptidne knjižnice;ELISA;
Affinity chromatography is the linchpin of antibody downstream processing and typically relies on bacterial immunoglobulin (Ig)-binding proteins, epitomized by staphylococcal protein A-based ligands. However, such afnity ligands are fairly costly and sufer from chemical instability, leading to ligan ...
Leto:
2021
Vir:
Fakulteta za farmacijo (UL FFA)
Izvirni znanstveni članek
Oznake:
thiazoles;hit profiling;promiscuous compounds;frequent hitters;privileged scaffolds;fragments;
Thiazoles exhibit a wide range of biological activities and therefore represent useful and attractive building blocks. To evaluate their usefulness and pinpoint their liabilities in fragment screening campaigns, we assembled a focused library of 49 fragment-sized thiazoles and thiadiazoles with vari ...
Leto:
2022
Vir:
Fakulteta za farmacijo (UL FFA)
Izvirni znanstveni članek
Oznake:
sinteza beljakovin;zaviralci Mycobacterium tuberculosis;imunoproteasom;protein degradation;proteasome;Mycobacterium tuberculosis;psoralens;nonpeptidic proteasome inhibitors;
Protein degradation is a fundamental process in all living organisms. An important part of this system is a multisubunit, barrel-shaped protease complex called the proteasome. This enzyme is directly responsible for the proteolysis of ubiquitin- or pup-tagged proteins to smaller peptides. In this st ...
Leto:
2020
Vir:
Fakulteta za farmacijo (UL FFA)