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Št. zadetkov: 7
Guidelines for the reliable use of high throughput sequencing technologies to detect plant pathogens and pests
Sébastien Massart, Ian Adams, Maher Al Rwahnih, Steve Baeyen, Guillaume J. Bilodeau, Arnaud G. Blouin, Neil Boonham, Thierry Candresse, Anne Chandellier, Kris De Jonghe, Denis Kutnjak, Nataša Mehle
Pregledni znanstveni članek
Oznake: bolezni rastlin;rastlinski virusi;rastlinski patogeni;rastlinski škodljivci;sekvenciranje;
High-throughput sequencing (HTS) technologies have the potential to become one of the most significant advances in molecular diagnostics. Their use by researchers to detect and characterize plant pathogens and pests has been growing steadily for more than a decade and they are now envisioned as a ro ...
Leto: 2022 Vir: Nacionalni inštitut za biologijo (NIB)
Biological and genetic characterization of Physostegia chlorotic mottle virus in Europe based on host range, location, and time
Coline Temple, Arnaud G. Blouin, Kris De Jonghe, Yoika Foucart, Marleen Botermans, Marcel Westenberg, Ruben Schoen, Pascal Gentit, Michèle Visage, Eric Verdin, Mark Paul Selda Rivarez, Denis Kutnjak, Ana Vučurović
Izvirni znanstveni članek
Oznake: biološka karakterizacija;genetska karakterizacija;PhCMoV;rastlinski virusi;
Application of high throughput sequencing (HTS) technologies enabled the first identification of Physostegia chlorotic mottle virus (PhCMoV) in 2018 in Austria. Subsequently, PhCMoV was detected in Germany and Serbia on tomatoes showing severe fruit mottling and ripening anomalies. We report here ho ...
Leto: 2022 Vir: Nacionalni inštitut za biologijo (NIB)
Looking beyond virus detection in RNA sequencing data
Annelies Haegeman, Yoika Foucart, Kris De Jonghe, Thomas Goedefroit, Maher Al Rwahnih, Neil Boonham, Thierry Candresse, Yahya Gaafar, Oscar Hurtado-Gonzales, Zala Kogej, Denis Kutnjak, Janja Lamovšek, Irena Mavrič Pleško
Izvirni znanstveni članek
Oznake: rastlinski patogeni;diagnostika;sekvenciranje;metagenomika;metatranskriptomatika;plant viruses;plant virus detection;plant virology;high-throughput sequencing;RNA sequencing;plant tissues;plant pathogen;diagnostics;metagenomics;metatranscriptomics;
High-throughput sequencing (HTS), more specifically RNA sequencing of plant tissues, has become an indispensable tool for plant virologists to detect and identify plant viruses. During the data analysis step, plant virologists typically compare the obtained sequences to reference virus databases. In ...
Leto: 2023 Vir: Nacionalni inštitut za biologijo (NIB)
Validation of molecular tests for the detection of tomato brown rugose fruit virus (ToBRFV) in seed of tomato and pepper
Anne Giesbers, Annelien J. W. Roenhorst, Martijn Schenk, Ruud Barnhoorn, Laura Tomassoli, Marta Luigi, Kris De Jonghe, Laëtitia Porcher, Pascal Gentit, Heiko Ziebell, Mouhammad Zeidan, Dorit Shargil, Sabine Grausgruber Groeger, Nataša Mehle
Končno poročilo o rezultatih raziskav
Oznake: virus rjave grbančavosti plodov paradižnika;medlaboratorijska primerjava testov;semena;
Tomato brown rugose fruit virus (ToBRFV) is a relatively recently described tobamovirus (Salem et al., 2016) causing problems in tomato and pepper cultivation worldwide (Luria et al., 2017, Salem et al., 2019). ToBRFV has been reported to cause yellow spots, green spots, necrotic lesions and occasio ...
Leto: 2021 Vir: Nacionalni inštitut za biologijo (NIB)
Interlaboratory comparison study on ribodepleted total RNA high-throughput sequencing for plant virus diagnostics and bioinformatic competence
Yahya Gaafar, Marcel Westenberg, Marleen Botermans, László Krizbai, Kris De Jonghe, Yoika Foucart, Luca Ferretti, Denis Kutnjak, Anja Pecman, Nataša Mehle, Jan F. Kreuze, Giovanna Müller, Nikolaos Vakirlis, Despoina Beris, Christina Varveri, Heiko Ziebell
Izvirni znanstveni članek
Oznake: high-throughput sequencing;ribodepletion;interlaboratory comparison;test performance study;proficiency test;Virtool;
High-throughput sequencing (HTS) technologies and bioinformatic analyses are of growing interest to be used as a routine diagnostic tool in the field of plant viruses. The reliability of HTS workflows from sample preparation to data analysis and results interpretation for plant virus detection and i ...
Leto: 2021 Vir: Nacionalni inštitut za biologijo (NIB)
ǂA ǂprimer on the analysis of high-throughput sequencing data for detection of plant viruses
Denis Kutnjak, Lucie Tamisier, Ian Adams, Neil Boonham, Thierry Candresse, Michela Chiumenti, Kris De Jonghe, Jan F. Kreuze, Marie Lefebvre, Goncalo Silva, Martha Malapi-Wight, Paolo Margaria, Irena Mavrič Pleško, Sam McGreig, Laura Miozzi, Benoit Remenant, Jean-Sébastien Reynard, Johan Rollin, Mike Rott, Olivier Schumpp, Sébastien Massart, Annelies Haegeman
Pregledni znanstveni članek
Oznake: varstvo rastlin;rastlinski virus;bioinformatika;mikroorganizmi;zaznavanje;odkritje;plant virus;high-throughput sequencing;bioinformatics;detection;discovery;
High-throughput sequencing (HTS) technologies have become indispensable tools assisting plant virus diagnostics and research thanks to their ability to detect any plant virus in a sample without prior knowledge. As HTS technologies are heavily relying on bioinformatics analysis of the huge amount of ...
Leto: 2021 Vir: Nacionalni inštitut za biologijo (NIB)
ǂA ǂset of experimentally validated decoys for the human CC chemokine receptor 7 (CCR7) obtained by virtual screening
Matic Proj, Steven De Jonghe, Tom Van Loy, Marko Jukič, Anže Meden, Luka Ciber, Črtomir Podlipnik, Uroš Grošelj, Janez Konc, Dominique Schols, Stanislav Gobec
Izvirni znanstveni članek
Oznake: virtual screening;decoys;chemical library;computer-aided drug design;CCR7;GPCR;
We present a state-of-the-art virtual screening workflow aiming at the identification of novel CC chemokine receptor 7 (CCR7) antagonists. Although CCR7 is associated with a variety of human diseases, such as immunological disorders, inflammatory diseases, and cancer, this target is underexplored in ...
Leto: 2022 Vir: Fakulteta za farmacijo (UL FFA)
Št. zadetkov: 7
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