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Št. zadetkov: 9
Use of a real-time LAMP isothermal assay for detecting 16SrII and XII phytoplasmas in fruit and weeds of the Ethiopian Rift Valley
B. Bekele, Janet Hodgetts, J. Tomlinson, Neil Boonham, Petra Nikolić, Philip Swarbrick, M. Dickinson
Izvirni znanstveni članek
Oznake: citrus;diagnostics (biology);LAMP assay;papaya;
Use of a real-time LAMP isothermal assay for detecting 16SrII and XII phytoplasmas in fruit and weeds of the Ethiopian Rift Valley
Leto: 2011 Vir: Repozitorij Univerze v Ljubljani (RUL)
Next generation sequencing for detection and discovery of plant viruses and viroids
Anja Pecman, Denis Kutnjak, Ion Gutierrez-Aguirre, Ian Adams, Adrian Fox, Neil Boonham, Maja Ravnikar
Izvirni znanstveni članek
Oznake: next generation sequencing;small RNA;ribosomal RNA depleted total RNA;detection;plant viruses;plant viroids;
Next generation sequencing (NGS) technologies are becoming routinely employed in different fields of virus research. Different sequencing platforms and sample preparation approaches, in the laboratories worldwide, contributed to a revolution in detection and discovery of plant viruses and viroids. I ...
Leto: 2017 Vir: Nacionalni inštitut za biologijo (NIB)
Aggressive and mild Potato virus Y isolates trigger different specific responses in susceptible potato plants
Polona Kogovšek, Maruša Pompe Novak, Špela Baebler, Ana Rotter, Lisa Gow, Kristina Gruden, Gary D. Foster, Neil Boonham, Maja Ravnikar
Izvirni znanstveni članek
Oznake: gene expression;microarray;quantitative real-time PCR;symptomatology;
Aggressive and mild Potato virus Y isolates trigger different specific responses in susceptible potato plants
Leto: 2010 Vir: Digitalni repozitorij raziskovalnih organizacij Slovenije
Highly specific qPCR and amplicon sequencing method for detection of quarantine citrus pathogen Phyllosticta citricarpaapplicable for air samples
Janja Zajc, Zala Kogej, Sara Fišer, Cene Gostinčar, Antonio Vicent, Anaïs Galvañ Domenech, Luca Riccioni, Neil Boonham, Maja Ravnikar, Polona Kogovšek
Izvirni znanstveni članek
Oznake: Phyllosticta citricarpa;detekcija;citrusi;zrak;detection;fungal spore sampling;internal transcribed region (ITS);translation elongation factor 1-α (TEF1);
The fungus Phyllosticta citricarpa is a quarantine pathogen in the EU and is of high economic importance in many parts of the world where favourable climate conditions drive the development of citrus black spot (CBS) disease. Disease symptoms include necrotic lesions on leaves and fruits. Low diseas ...
Leto: 2023 Vir: Nacionalni inštitut za biologijo (NIB)
High-throughput sequencing facilitates characterisation of a ʺforgottenʺ plant virus
Anja Pecman, Denis Kutnjak, Nataša Mehle, Magda Tušek-Žnidarič, Ion Gutierrez-Aguirre, Patricija Pirnat, Ian Adams, Neil Boonham, Maja Ravnikar
Izvirni znanstveni članek
Oznake: henbane mosaic virus;Tomato;high-throughput sequencing;host range analysis;phylogeny;Potyvirus;
High-throughput sequencing has dramatically broadened the possibilities for plant virus research and diagnostics, enabling discovery of new or obscure viruses, and virus strains and rapid sequencing of their genomes. In this research, we employed high-throughput sequencing to discover a new virus in ...
Leto: 2018 Vir: Nacionalni inštitut za biologijo (NIB)
LAMP assay and rapid sample preparation method for on-site detection of flavescence dorée phytoplasma in grapevine
Polona Kogovšek, Jennifer Hodgetts, J. Hall, Nina Prezelj, Petra Nikolić, Nataša Mehle, Rok Lenarčič, Ana Rotter, M. Dickinson, Neil Boonham, Marina Dermastia, Maja Ravnikar
Izvirni znanstveni članek
Oznake: flavescence dorée;homogenization;loop-mediated isothermal amplification;on-site application;validation;
In Europe the most devastating phytoplasma associated with grapevine yellows (GY) diseases is a quarantine pest, flavescence dorée (FDp), from the 16SrV taxonomic group. The on-site detection of FDp with an affordable device would contribute to faster and more efficient decisions on the control meas ...
Leto: 2015 Vir: Nacionalni inštitut za biologijo (NIB)
Guidelines for the reliable use of high throughput sequencing technologies to detect plant pathogens and pests
Sébastien Massart, Ian Adams, Maher Al Rwahnih, Steve Baeyen, Guillaume J. Bilodeau, Arnaud G. Blouin, Neil Boonham, Thierry Candresse, Anne Chandellier, Kris De Jonghe, Denis Kutnjak, Nataša Mehle
Pregledni znanstveni članek
Oznake: bolezni rastlin;rastlinski virusi;rastlinski patogeni;rastlinski škodljivci;sekvenciranje;
High-throughput sequencing (HTS) technologies have the potential to become one of the most significant advances in molecular diagnostics. Their use by researchers to detect and characterize plant pathogens and pests has been growing steadily for more than a decade and they are now envisioned as a ro ...
Leto: 2022 Vir: Nacionalni inštitut za biologijo (NIB)
Looking beyond virus detection in RNA sequencing data
Annelies Haegeman, Yoika Foucart, Kris De Jonghe, Thomas Goedefroit, Maher Al Rwahnih, Neil Boonham, Thierry Candresse, Yahya Gaafar, Oscar Hurtado-Gonzales, Zala Kogej, Denis Kutnjak, Janja Lamovšek, Irena Mavrič Pleško
Izvirni znanstveni članek
Oznake: rastlinski patogeni;diagnostika;sekvenciranje;metagenomika;metatranskriptomatika;plant viruses;plant virus detection;plant virology;high-throughput sequencing;RNA sequencing;plant tissues;plant pathogen;diagnostics;metagenomics;metatranscriptomics;
High-throughput sequencing (HTS), more specifically RNA sequencing of plant tissues, has become an indispensable tool for plant virologists to detect and identify plant viruses. During the data analysis step, plant virologists typically compare the obtained sequences to reference virus databases. In ...
Leto: 2023 Vir: Nacionalni inštitut za biologijo (NIB)
ǂA ǂprimer on the analysis of high-throughput sequencing data for detection of plant viruses
Denis Kutnjak, Lucie Tamisier, Ian Adams, Neil Boonham, Thierry Candresse, Michela Chiumenti, Kris De Jonghe, Jan F. Kreuze, Marie Lefebvre, Goncalo Silva, Martha Malapi-Wight, Paolo Margaria, Irena Mavrič Pleško, Sam McGreig, Laura Miozzi, Benoit Remenant, Jean-Sébastien Reynard, Johan Rollin, Mike Rott, Olivier Schumpp, Sébastien Massart, Annelies Haegeman
Pregledni znanstveni članek
Oznake: varstvo rastlin;rastlinski virus;bioinformatika;mikroorganizmi;zaznavanje;odkritje;plant virus;high-throughput sequencing;bioinformatics;detection;discovery;
High-throughput sequencing (HTS) technologies have become indispensable tools assisting plant virus diagnostics and research thanks to their ability to detect any plant virus in a sample without prior knowledge. As HTS technologies are heavily relying on bioinformatics analysis of the huge amount of ...
Leto: 2021 Vir: Nacionalni inštitut za biologijo (NIB)
Št. zadetkov: 9
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