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Št. zadetkov: 4
Assessing nuclear versus mitochondrial cell-free DNA (cfDNA) by qRT-PCR and droplet digital PCR using a piglet model of perinatal asphyxia
Marie Bitenc, Benedicte Grebstad Tune, Maria Melheim, Monica Atneosen‑Åsegg, Polona Rajar
Izvirni znanstveni članek
Oznake: cell-free DNA;digital PCR;mitochondrial cfDNA;nuclear cfDNA;perinatal asphyxia;qRT-PCR;
Background: Since the discovery more than half a century ago, cell-free DNA (cfDNA) has become an attractive objective in multiple diagnostic, prognostic, and monitoring settings. However, despite the increasing number of cfDNA applications in liquid biopsies, we still lack a comprehensive understan ...
Leto: 2023 Vir: Digitalni repozitorij raziskovalnih organizacij Slovenije
Antibiotic stewardship in premature infants
Polona Rajar, Ola Didrik Saugstad, Dag Berild, Anirban Dutta, Gorm Greisen, Ulrik Lausten-Thomsen
Pregledni znanstveni članek
Oznake: antibiotic resistance;antibiotic stewardship;premature infant;
Introduction: Antibiotic treatment in premature infants is often empirically prescribed, and practice varies widely among otherwise comparable neonatal intensive care units. Unnecessary and prolonged antibiotic treatment is documented in numerous studies. Recent research shows serious side effects a ...
Leto: 2021 Vir: Digitalni repozitorij raziskovalnih organizacij Slovenije
Prolonged hospitalization signature and early antibiotic effects on the nasopharyngeal resistome in preterm infants
Achal Dhariwal, Polona Rajar, Gabriela Salvadori, Heidi Aarø Åmdal, Dag Berild, Ola Didrik Saugstad, Drude Fugelseth, Gorm Greisen
Izvirni znanstveni članek
Oznake: preterm infants;respiratory resistome;whole genome sequencing;genetics;drug effects;
Respiratory pathogens, commonly colonizing nasopharynx, are among the leading causes of death due to antimicrobial resistance. Yet, antibiotic resistance determinants within nasopharyngeal microbial communities remain poorly understood. In this prospective cohort study, we investigate the nasopharyn ...
Leto: 2024 Vir: Digitalni repozitorij raziskovalnih organizacij Slovenije
Microbial DNA extraction of high-host content and low biomass samples
Polona Rajar, Achal Dhariwal, Gabriela Salvadori, Roger Junges, Heidi Aarø Åmdal, Dag Berild, Drude Fugelseth, Ola Didrik Saugstad
Izvirni znanstveni članek
Oznake: antimicrobial resistance;host DNA depletion;low biomass;microbiome;premature infant;resistome;respiratory microbiome;whole metagenomic sequencing;
Introduction: Low microbial biomass and high human DNA content in nasopharyngeal aspirate samples hinder comprehensive characterization of microbiota and resistome. We obtained samples from premature infants, a group with increased risk of developing respiratory disorders and infections, and consequ ...
Leto: 2022 Vir: Digitalni repozitorij raziskovalnih organizacij Slovenije
Št. zadetkov: 4
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