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Microbial DNA extraction of high-host content and low biomass samples
Polona Rajar, Achal Dhariwal, Gabriela Salvadori, Roger Junges, Heidi Aarø Åmdal, Dag Berild, Drude Fugelseth, Ola Didrik Saugstad
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Oznake: antimicrobial resistance;host DNA depletion;low biomass;microbiome;premature infant;resistome;respiratory microbiome;whole metagenomic sequencing;
Introduction: Low microbial biomass and high human DNA content in nasopharyngeal aspirate samples hinder comprehensive characterization of microbiota and resistome. We obtained samples from premature infants, a group with increased risk of developing respiratory disorders and infections, and consequ ...
Leto: 2022 Vir: Digitalni repozitorij raziskovalnih organizacij Slovenije
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