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Št. zadetkov: 3
Revisiting the role of pulsed electric fields in overcoming the barriers to in vivo gene electrotransfer
Shaurya Sachdev, Tjaša Potočnik, Lea Rems, Damijan Miklavčič
Pregledni znanstveni članek
Oznake: elektroporacija;genska terapija;genska transfekcija;electroporation;gene medicine;gene transfection;
Gene therapies are revolutionizing medicine by providing a way to cure hitherto incurable diseases. The scientific and technological advances have enabled the first gene therapies to become clinically approved. In addition, with the ongoing COVID-19 pandemic, we are witnessing record speeds in the d ...
Leto: 2022 Vir: Fakulteta za elektrotehniko (UL FE)
Efficient gene transfection by electroporation - in vitro and in silico study of pulse parameters
Tjaša Potočnik, Shaurya Sachdev, Tamara Polajžer, Alenka Maček Lebar, Damijan Miklavčič
Izvirni znanstveni članek
Oznake: genski elektroprenos;nanosekundni pulzi;visokofrekvenčni bipolarni pulzi;elektroporacija;plazmidna DNA;gene electrotransfer;nanosecond pulses;high frequency bipolar pulses;electroporation;plasmid DNA;
Gene electrotransfer (GET) is a widely used method for nucleic acids’ delivery into cells. We explored, evaluated, and demonstrated the potential use of different pulse durations for introducing plasmid DNA (pDNA) into cells in vitro and compared the efficiency and dynamics of transgene expression a ...
Leto: 2022 Vir: Fakulteta za elektrotehniko (UL FE)
Gene electrotransfer into mammalian cells using commercial cell culture inserts with porous substrate
Tina Cimperman, Anja Blažič, Diaa Khayyat, Tjaša Potočnik, Shaurya Sachdev, Lea Rems
Izvirni znanstveni članek
Oznake: lokalizirana elektroporacija;genska elektrotransfekcija;transfekcija;vstavek za mikrotitrsko ploščo;numerično modeliranje;ovarijske celice kitajskega hrčka;celična linija C2C12;celična linija H9C2;localized electroporation;gene electrotransfer;plasmid;transfection;cell structure insert;numerical modeling;Chinese hamster ovary cells;myoblasts;C2C12 cell line;H9C2 cell line;
Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane transport. By localizing the electric field over small portions of the cell memb ...
Leto: 2022 Vir: Fakulteta za elektrotehniko (UL FE)
Št. zadetkov: 3
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