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Controlling in vitro mRNA polyadenylation by monitoring poly(A) polymerase consumption of ATP
Janja Skok, Pooja Tiwari, Tina Vodopivec, Sergeja Lebar, Ana Ferjančič, Anže Martinčič Celjar, Polona Megušar, Matija Povh, Nina Mencin, Swapnil Bawage, Shree Ram Singh, Artem V. Badasyan, Rok Sekirnik
Izvirni znanstveni članek
Oznake: mRNA;HPLC;poly(A) polymerase (PAP);polyadenylation, poly(A) tail;in-process analytics;
The poly(A) tail of mRNA plays a vital role in mRNA transcript stability, translational efficiency, and immunogenicity. Co-transcriptionally polyadenylated in vitro transcribed (IVT) mRNAs typically contain poly(A) tails of 50–120 nucleotide tail length due to limitations in production of template p ...
Leto: 2026 Vir: Univerza v Novi Gorici (UNG)
Št. zadetkov: 1
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