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Št. zadetkov: 2
Excitation and electroporation in genetically engineered excitable S-HEK cells exposed to electric pulses of different durations
Tina Batista Napotnik, Tina Cimperman, Lea Rems
Izvirni znanstveni članek
Oznake: elektroporacija;vzdražne celice;akcijski potencial;dolžina pulza;znotrajcelični kalcij;electroporation;exitable cells;action potential;pulse duration;intracellular calcium;
Electroporation affects action potential generation in excitable cells such as nerve, muscle and cardiac cells. Since electroporation-based treatments use different pulse protocols, we investigated how electric pulses of different duration (from 500 ns to 1 ms) trigger action potentials and cause el ...
Leto: 2025 Vir: Fakulteta za elektrotehniko (UL FE)
Gene electrotransfer into mammalian cells using commercial cell culture inserts with porous substrate
Tina Cimperman, Anja Blažič, Diaa Khayyat, Tjaša Potočnik, Shaurya Sachdev, Lea Rems
Izvirni znanstveni članek
Oznake: lokalizirana elektroporacija;genska elektrotransfekcija;transfekcija;vstavek za mikrotitrsko ploščo;numerično modeliranje;ovarijske celice kitajskega hrčka;celična linija C2C12;celična linija H9C2;localized electroporation;gene electrotransfer;plasmid;transfection;cell structure insert;numerical modeling;Chinese hamster ovary cells;myoblasts;C2C12 cell line;H9C2 cell line;
Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane transport. By localizing the electric field over small portions of the cell memb ...
Leto: 2022 Vir: Fakulteta za elektrotehniko (UL FE)
Št. zadetkov: 2
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