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Št. zadetkov: 3
Biological and genetic characterization of Physostegia chlorotic mottle virus in Europe based on host range, location, and time
Coline Temple, Arnaud G. Blouin, Kris De Jonghe, Yoika Foucart, Marleen Botermans, Marcel Westenberg, Ruben Schoen, Pascal Gentit, Michèle Visage, Eric Verdin, Mark Paul Selda Rivarez, Denis Kutnjak, Ana Vučurović
Izvirni znanstveni članek
Oznake: biološka karakterizacija;genetska karakterizacija;PhCMoV;rastlinski virusi;
Application of high throughput sequencing (HTS) technologies enabled the first identification of Physostegia chlorotic mottle virus (PhCMoV) in 2018 in Austria. Subsequently, PhCMoV was detected in Germany and Serbia on tomatoes showing severe fruit mottling and ripening anomalies. We report here ho ...
Leto: 2022 Vir: Nacionalni inštitut za biologijo (NIB)
Looking beyond virus detection in RNA sequencing data
Annelies Haegeman, Yoika Foucart, Kris De Jonghe, Thomas Goedefroit, Maher Al Rwahnih, Neil Boonham, Thierry Candresse, Yahya Gaafar, Oscar Hurtado-Gonzales, Zala Kogej, Denis Kutnjak, Janja Lamovšek, Irena Mavrič Pleško
Izvirni znanstveni članek
Oznake: rastlinski patogeni;diagnostika;sekvenciranje;metagenomika;metatranskriptomatika;plant viruses;plant virus detection;plant virology;high-throughput sequencing;RNA sequencing;plant tissues;plant pathogen;diagnostics;metagenomics;metatranscriptomics;
High-throughput sequencing (HTS), more specifically RNA sequencing of plant tissues, has become an indispensable tool for plant virologists to detect and identify plant viruses. During the data analysis step, plant virologists typically compare the obtained sequences to reference virus databases. In ...
Leto: 2023 Vir: Nacionalni inštitut za biologijo (NIB)
Interlaboratory comparison study on ribodepleted total RNA high-throughput sequencing for plant virus diagnostics and bioinformatic competence
Yahya Gaafar, Marcel Westenberg, Marleen Botermans, László Krizbai, Kris De Jonghe, Yoika Foucart, Luca Ferretti, Denis Kutnjak, Anja Pecman, Nataša Mehle, Jan F. Kreuze, Giovanna Müller, Nikolaos Vakirlis, Despoina Beris, Christina Varveri, Heiko Ziebell
Izvirni znanstveni članek
Oznake: high-throughput sequencing;ribodepletion;interlaboratory comparison;test performance study;proficiency test;Virtool;
High-throughput sequencing (HTS) technologies and bioinformatic analyses are of growing interest to be used as a routine diagnostic tool in the field of plant viruses. The reliability of HTS workflows from sample preparation to data analysis and results interpretation for plant virus detection and i ...
Leto: 2021 Vir: Nacionalni inštitut za biologijo (NIB)
Št. zadetkov: 3
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